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1. (WO2019028381) METHODS FOR LYSIS OF CELLS WITHIN A SAMPLE
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Pub. No.: WO/2019/028381 International Application No.: PCT/US2018/045211
Publication Date: 07.02.2019 International Filing Date: 03.08.2018
IPC:
C12N 15/10 (2006.01) ,C12N 15/11 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
Applicants:
MICROBEDX, INC. [US/US]; 17866 Tramonto Drive Pacific Palisades, California 90272, US
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA [US/US]; 1111 Franklin Street, Twelfth Floor Oakland, California 94607-5200, US
Inventors:
MONTI, Gabriel; US
HALFORD, Colin, Wynn; US
PEREBIKOVSKY, Alexandra; US
LIU, Yujia; US
KIDO, Horacio; US
HAAKE, David, Arnold; US
MADOU, Marc; US
GUSSIN, Daniel; US
CHURCHILL, Bernard; US
Agent:
TOMSA, Michael, S.; US
Priority Data:
62/541,41804.08.2017US
Title (EN) METHODS FOR LYSIS OF CELLS WITHIN A SAMPLE
(FR) PROCÉDÉS DE LYSE DE CELLULES DANS UN ÉCHANTILLON
Abstract:
(EN) There is described a method for extracting a target chemical compound from a cellular material in a sample. The method comprising the steps of: subjecting the sample to mechanical lysis to cause disruption of a cellular membrane in the cellular material; contacting the sample with an alkaline material to produce a lysate composition comprising the target chemical compound; and recovering the lysate composition from the sample. There is also described a method for producing a lysate composition comprising RNA from a mammalian bodily fluid sample comprising a cellular material. There is also described a method for extracting a nucleic acid from a cellular material in a bodily fluid or an inoculant derived therefrom.
(FR) L'invention concerne un procédé d'extraction d'un composé chimique cible à partir d'un matériel cellulaire dans un échantillon. Le procédé comprend les étapes consistant : à soumettre l'échantillon à une lyse mécanique pour provoquer la rupture d'une membrane cellulaire dans le matériel cellulaire ; à mettre en contact l'échantillon avec une matière alcaline pour produire une composition de lysat comprenant le composé chimique cible ; et à récupérer la composition de lysat à partir de l'échantillon. L'invention concerne également un procédé de production d'une composition de lysat comprenant de l'ARN à partir d'un échantillon de fluide corporel de mammifère contenant un matériel cellulaire. L'invention concerne également un procédé d'extraction d'un acide nucléique à partir d'un matériel cellulaire dans un fluide corporel ou d'un inoculant dérivé de ce dernier.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)