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1. (WO2019028131) NICHES-ON-A-CHIP
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Pub. No.: WO/2019/028131 International Application No.: PCT/US2018/044791
Publication Date: 07.02.2019 International Filing Date: 01.08.2018
IPC:
B01L 3/00 (2006.01) ,C12N 5/00 (2006.01)
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
L
CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3
Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
Applicants:
WAKE FOREST UNIVERSITY HEALTH SCIENCES [US/US]; Medical Center Blvd. Winston-Salem, North Carolina 27157, US
Inventors:
PORADA, Christopher Daniel; US
SKARDAL, Aleksander; US
ALMEIDA-PORADA, Maria Graca; US
Agent:
MURPHY, Sherry L.; US
Priority Data:
62/540,39102.08.2017US
62/571,98813.10.2017US
Title (EN) NICHES-ON-A-CHIP
(FR) NICHES SUR PUCE
Abstract:
(EN) Provided herein according to some embodiments is an in vitro construct useful as a model for a hematopoietic microenvironment, which may include: a microfluidic device having multiple chambers; and two or more populations of cells (e.g., 3 or 4 populations of cells) (or "niches") selected from: 1) mesenchymal cells (e.g., Stro-1+; MSC); 2) osteoblasts (OB; optionally said osteoblasts provided by differentiating mesenchymal cells to differentiated osteoblasts); 3) arterial endothelium (e.g., CD146+NG2+; AEC); and 4) sinusoidal endothelium (CD146+NG2-; SEC), wherein each of said two or more populations of cells are provided in a separate chamber of the microfluidic device. Methods of making and using the construct are also provided.
(FR) Selon certains modes de réalisation, la présente invention concerne une construction in vitro utile en tant que modèle pour un micro-environnement hématopoïétique, qui peut comprendre : un dispositif microfluidique ayant de multiples chambres ; et au moins deux populations de cellules ( par exemple , 3 ou 4 populations de cellules) (ou "niches") choisies parmi : 1) des cellules mésenchymateuses (par exemple Stro -1 +; MSC); 2) des ostéoblastes (OB; éventuellement lesdits ostéoblastes obtenus par différenciation de cellules mésenchymateuses en ostéoblastes différenciés); 3) l'endothélium artériel (par exemple CD146+NG2+; AEC); et 4) l'endothélium sinusoïdal (CD146+NG2-; SEC), chacune desdites au moins deux populations de cellules étant disposée dans une chambre séparée du dispositif microfluidique. L’invention concerne en outre des procédés de fabrication et d’utilisation de la construction.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)