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1. (WO2019028047) SPATIAL INDEXING OF GENETIC MATERIAL AND LIBRARY PREPARATION USING HYDROGEL BEADS AND FLOW CELLS
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Pub. No.: WO/2019/028047 International Application No.: PCT/US2018/044646
Publication Date: 07.02.2019 International Filing Date: 31.07.2018
IPC:
C12Q 1/68 (2018.01) ,C12Q 1/6874 ,B01L 3/00 (2006.01) ,C12Q 1/6869
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
[IPC code unknown for C12Q 1/6874]
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
L
CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3
Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
[IPC code unknown for C12Q 1/6869]
Applicants:
ILLUMINA, INC [US/US]; 5200 Illumina Way San Diego, CA 92122, US
Inventors:
KHURANA, Tarun, Kumar; US
WU, Yir-Shyuan; US
CHEN, Xi-Jun; US
GORPE-YASAR, Filiz; US
LIN, Yan-You; US
POPIC, Victoria; US
JAEGER, Erich, B.; US
RONAGHI, Mostafa; US
Agent:
WU, Bruce; US
Priority Data:
62/539,94901.08.2017US
62/663,12926.04.2018US
Title (EN) SPATIAL INDEXING OF GENETIC MATERIAL AND LIBRARY PREPARATION USING HYDROGEL BEADS AND FLOW CELLS
(FR) INDEXATION SPATIALE DE MATÉRIEL GÉNÉTIQUE ET PRÉPARATION DE PHARMACOTHÈQUE À L'AIDE DE BILLES D'HYDROGEL ET DE CELLULES D'ÉCOULEMENT
Abstract:
(EN) Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.
(FR) L'invention concerne des modes de réalisation d'un procédé d'ensemencement de pharmacothèques de séquences sur une surface d'une cellule d'écoulement de séquençage, qui permettent une ségrégation spatiale des pharmacothèques sur la surface. La ségrégation spatiale peut servir à indexer des lectures de séquence à partir de pharmacothèques individuelles de séquençage, pour augmenter l'efficacité d'une analyse ultérieure de données. Dans certains exemples, des billes d'hydrogel contenant des pharmacothèques encapsulées de séquençage sont capturées sur une cellule d'écoulement de séquençage et dégradées en présence d'une barrière de diffusion de liquide pour permettre la ségrégation spatiale et l'ensemencement des pharmacothèques de séquençage à la surface de la cellule d'écoulement. De plus, l'invention concerne des exemples de systèmes, de procédés et de compositions concernant des dispositifs à cellules d'écoulement conçus pour la préparation de pharmacothèque d'acides nucléiques et pour le séquençage de cellules individuelles. Certains exemples comprennent des dispositifs à cellules d'écoulement contenant un hydrogel où se trouve du matériel génétique et qui est retenu à l'intérieur de l'hydrogel pendant un traitement d'acides nucléiques.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)