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1. (WO2019028029) ASSESSMENT OF CRISPR/CAS-INDUCED RECOMBINATION WITH AN EXOGENOUS DONOR NUCLEIC ACID IN VIVO
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Pub. No.: WO/2019/028029 International Application No.: PCT/US2018/044612
Publication Date: 07.02.2019 International Filing Date: 31.07.2018
IPC:
A01K 67/027 (2006.01) ,C12N 15/90 (2006.01)
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
K
ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
67
Rearing or breeding animals, not otherwise provided for; New breeds of animals
027
New breeds of vertebrates
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
87
Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
90
Stable introduction of foreign DNA into chromosome
Applicants:
REGENERON PHARMACEUTICALS, INC. [US/US]; 777 Old Saw Mill River Road Tarrytown, New York 10591, US
Inventors:
GONG, Guochun; US
HUNT, Charleen; US
HARTFORD, Suzanne; US
ROJAS, Jose; US
FRENDEWEY, David; US
ZAMBROWICZ, Brian; US
MURPHY, Andrew J.; US
Agent:
SCHILLING, Stephen H.; US
LIEBESCHUETZ, Joe; US
CELLI, Rosemarie; US
SATHER, Susan; US
O'CONNOR, Daniel J.; US
HIGBEE, Zachary A.; US
CHANG, Cindy; US
Priority Data:
62/539,28531.07.2017US
Title (EN) ASSESSMENT OF CRISPR/CAS-INDUCED RECOMBINATION WITH AN EXOGENOUS DONOR NUCLEIC ACID IN VIVO
(FR) ÉVALUATION DE LA RECOMBINAISON INDUITE PAR CRISPR/CAS AVEC UN ACIDE NUCLÉIQUE DONNEUR EXOGÈNE IN VIVO
Abstract:
(EN) Methods and compositions are provided for assessing CRISPR/Cas-induced recombination of a target genomic locus with an exogenous donor nucleic acid in vivo or ex vivo. The methods and compositions employ non-human animals comprising a CRISPR reporter such as a genomically integrated CRISPR reporter for detecting and measuring CRISPR/Cas-induced repair of a coding sequence for a catalytically inactive reporter protein through recombination with an exogenous donor nucleic acid. Methods and compositions are also provided for making and using these non-human animals.
(FR) La présente invention concerne des procédés et des compositions pour évaluer la recombinaison induite par CRISPR/Cas d'un locus génomique cible avec un acide nucléique donneur exogène in vivo ou ex vivo. Les procédés et compositions utilisent des animaux non humains comprenant un rapporteur CRISPR tel qu'un rapporteur CRISPR génomiquement intégré pour détecter et mesurer une réparation induite par CRISPR/Cas d'une séquence codante pour une protéine rapporteur catalytiquement inactive par recombinaison avec un acide nucléique donneur exogène. L'invention concerne également des procédés et des compositions pour fabriquer et utiliser ces animaux non humains.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)