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1. (WO2019027861) METHODS AND COMPOSITIONS FOR VIRAL-BASED GENE EDITING IN PLANTS
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Pub. No.: WO/2019/027861 International Application No.: PCT/US2018/044285
Publication Date: 07.02.2019 International Filing Date: 30.07.2018
IPC:
A01H 1/06 (2006.01) ,A24B 13/00 (2006.01) ,A01H 5/12 (2018.01) ,C12N 15/82 (2006.01)
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
H
NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
1
Processes for modifying genotypes
06
Processes for producing mutations, e.g. treatment with chemicals or with radiation
A HUMAN NECESSITIES
24
TOBACCO; CIGARS; CIGARETTES; SMOKERS' REQUISITES
B
MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
13
Tobacco for pipes, for cigars, e.g. cigar inserts, or for cigarettes; Chewing tobacco; Snuff
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
H
NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5
Flowering plants, i.e. angiosperms
12
Leaves
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
82
for plant cells
Applicants:
R. J. REYNOLDS TOBACCO COMPANY [US/US]; 401 North Main Street Winston-salem, North Carolina 27101, US
Inventors:
LI, Xingpeng; US
HE, Yijian; US
POGUE, Gregory P.; US
HIATT, Emmett Ernest; US
LAWSON, Darlene Madeline; US
Agent:
CALKINS, Charles W.; US
ROTHSCHILD, Cynthia B.; US
Priority Data:
62/539,16031.07.2017US
Title (EN) METHODS AND COMPOSITIONS FOR VIRAL-BASED GENE EDITING IN PLANTS
(FR) PROCÉDÉS ET COMPOSITIONS POUR L'ÉDITION DE GÈNES À BASE DE VIRUS DANS DES PLANTES
Abstract:
(EN) The present disclosure provides compositions and methods for editing a target site of a plant genome by delivery of functional editing components using modified tobacco mosaic virus (mTMV). The methods disclosed herein can be used to deliver a gene editing system, such as a DNA endonuclease, to a tobacco plant cell for modification of a target site of the plant genome. Further, the methods and compositions disclosed herein provide for production of a RNA molecule encoding a meganuclease in vitro prior to delivery of the RNA to a plant cell. After introduction of the nucleic acid molecule encoding a functional editing component and subsequent expression of the functional editing components, the plant can be cultured and allowed to produce seeds having an edit at a genomic target site. The seeds can then undergo embryo rescue and be cultured to produce a modified plant without heterologous genetic material.
(FR) La présente invention concerne des compositions et des procédés permettant d'éditer un site cible d'un génome de plante par l'administration de constituants d'édition fonctionnels à l'aide d'un virus modifié de la mosaïque du tabac (mTMV). Les procédés de l'invention peuvent être utilisés pour administrer un système d'édition de gène, tel qu'une ADN endonucléase, à une cellule de plant de tabac pour la modification d'un site cible du génome de plante. En outre, les procédés et les compositions de l'invention permettent la production d'une molécule d'ARN codant pour une méganucléase in vitro avant l'administration de l'ARN à une cellule végétale. Après l'introduction de la molécule d'acide nucléique codant pour un constituant d'édition fonctionnel et l'expression ultérieure des constituants d'édition fonctionnels, la plante peut être cultivée et peut produire des graines ayant un site cible génomique édité. Les graines peuvent ensuite subir un sauvetage d'embryon et être cultivées pour produire une plante modifiée sans matériel génétique hétérologue.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)