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1. (WO2019027298) PHARMACEUTICAL COMPOSITION COMPRISING MESENCHYMAL STEM CELL EXPRESSING TRAIL AND CD AS EFFECTIVE INGREDIENT FOR PREVENTION OR TREATMENT OF CANCER
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Pub. No.: WO/2019/027298 International Application No.: PCT/KR2018/008901
Publication Date: 07.02.2019 International Filing Date: 06.08.2018
IPC:
A61K 35/28 (2015.01) ,C07K 14/47 (2006.01) ,C12N 9/78 (2006.01) ,C12N 5/0775 (2010.01) ,C12N 15/86 (2006.01)
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35
Medicinal preparations containing material or reaction products thereof with undetermined constitution
12
Materials from mammals or birds
28
Marrow; Spleen
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
46
from vertebrates
47
from mammals
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
78
acting on carbon to nitrogen bonds other than peptide bonds (3.5)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
077
Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
0775
Mesenchymal stem cells; Adipose-tissue derived stem cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
86
Viral vectors
Applicants:
주식회사 에스엘바이젠 SLBIGEN INC. [KR/KR]; 경기도 성남시 분당구 대왕판교로 700 C동 7층 7F, Bldg. C, 700, Daewangpangyo-ro, Bundang-gu Seongnam-si, Gyeonggi-do 13488, KR
가톨릭대학교 산학협력단 THE CATHOLIC UNIVERSITY OF KOREA INDUSTRY-ACADEMIC COOPERATION FOUNDATION [KR/KR]; KR
Inventors:
전신수 JEUN, Sin-Soo; KR
이순민 LEE, Soon Min; KR
김혜연 KIM, Hey-yon; KR
Agent:
제일특허법인(유) FIRSTLAW P.C.; 서울시 서초구 마방로 60 60 Mabang-Ro Seocho-Ku Seoul 06775, KR
Priority Data:
10-2017-009915304.08.2017KR
Title (EN) PHARMACEUTICAL COMPOSITION COMPRISING MESENCHYMAL STEM CELL EXPRESSING TRAIL AND CD AS EFFECTIVE INGREDIENT FOR PREVENTION OR TREATMENT OF CANCER
(FR) COMPOSITION PHARMACEUTIQUE COMPRENANT UNE CELLULE SOUCHE MÉSENCHYMATEUSE EXPRIMANT TRAIL ET CD EN TANT QUE PRINCIPE ACTIF POUR LA PRÉVENTION OU LE TRAITEMENT DU CANCER
(KO) TRAIL 및 CD를 발현하는 중간엽줄기세포를 유효성분으로 포함하는 암의 예방또는 치료용 약학 조성물
Abstract:
(EN) The present invention relates to a pharmaceutical composition comprising mesenchymal stem cells expressing TRAIL and CD as an effective ingredient for prevention or treatment of cancer. A pharmaceutical composition comprising TRAIL- and CD-expressing mesenchymal stem cells as an effective ingredient for prevention or treatment of cancer according to the present invention induces cancer cells to undergo apoptosis, thereby inhibiting cancer cell formation and metastasis. In addition, as immortalized mesenchymal stem cells that have a high cell proliferation rate, the mesenchymal stem cells are unlikely to abnormally differentiate due to the stable expression of an introduced gene because the cells can regulate the expression of TRAIL protein and CD protein therein according to treatment with or without doxycycline, and are of high stability because apoptosis is induced by 5-FC treatment to allow the removal of residual stem cells. In addition, cell lines expressing both TRAIL and CD were observed to have excellent anticancer effects. Therefore, the pharmaceutical composition of the present invention can be effectively used for prevention or treatment of cancer.
(FR) La présente invention concerne une composition pharmaceutique comprenant des cellules souches mésenchymateuses exprimant TRAIL et CD en tant que principe actif pour la prévention ou le traitement du cancer. Une composition pharmaceutique comprenant des cellules souches mésenchymateuses exprimant TRAIL et CD en tant que principe actif pour la prévention ou le traitement du cancer selon la présente invention induit l'apoptose des cellules cancéreuses, ce qui permet d'inhiber la formation de cellules cancéreuses et la métastase. En outre, en tant que cellules souches mésenchymateuses immortalisées qui ont un taux de prolifération cellulaire élevé, les cellules souches mésenchymateuses sont peu susceptibles de se différencier anormalement du fait de l'expression stable d'un gène introduit car les cellules peuvent réguler leur expression de protéine TRAIL et de protéine CD en fonction d'un traitement avec ou sans doxycycline, et présentent une grande stabilité car l'apoptose est induite par un traitement 5-FC pour permettre l'élimination de cellules souches résiduelles. De plus, on a observé que des lignées cellulaires exprimant à la fois TRAIL et CD avaient d'excellents effets anticancéreux. Par conséquent, la composition pharmaceutique de la présente invention peut être utilisée efficacement pour la prévention ou le traitement du cancer.
(KO) 본 발명은 TRAIL 및 CD를 발현하는 중간엽줄기세포를 유효성분으로 포함 하는 암의 예방 또는 치료용 약학 조성물에 관한 것이다. 본 발명의 TRAIL 및 CD 를 발현하는 중간엽줄기세포를 유효성분으로 포함하는 암 예방 또는 치료용 약학 조성물은 암세포의 세포사멸을 유도함으로써 암세포의 형성 및 전이를 억제한다. 또한, 상기 중간엽줄기세포는 불사화된 중간엽줄기세포로서 높은 세포 증식율을 가지면서, 독시사이클린 처리 유무에 따라 세포 내에서 TRAIL 단백질 및 CD 단백 질의 발현을 조절할 수 있기 때문에 도입 유전자의 지속적인 발현으로 인한 비정 상적인 분화 가능성이 낮고, 5-FC 처리에 의해 세포사멸이 유도됨으로써 잔존 줄 기세포를 제거할 수 있어 안정성이 높다. 뿐만 아니라, TRAIL 및 CD를 동시에 발 현하는 세포주의 항암 효과가 우월함을 확인한 바, 본 발명의 약학 조성물은 암 의 예방 또는 치료에 유용하게 사용될 수 있다.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Korean (KO)
Filing Language: Korean (KO)