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1. (WO2019026976) MODIFIED Cas9 PROTEINS AND APPLICATION FOR SAME
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2019/026976 International Application No.: PCT/JP2018/028926
Publication Date: 07.02.2019 International Filing Date: 01.08.2018
IPC:
C12N 15/09 (2006.01) ,C12N 9/22 (2006.01) ,C12N 15/55 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
16
acting on ester bonds (3.1)
22
Ribonucleases
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
52
Genes encoding for enzymes or proenzymes
55
Hydrolases (3)
Applicants:
国立大学法人 東京大学 THE UNIVERSITY OF TOKYO [JP/JP]; 東京都文京区本郷七丁目3番1号 3-1, Hongo 7-chome, Bunkyo-ku, Tokyo 1138654, JP
エディジーン株式会社 EDIGENE CORPORATION [JP/JP]; 東京都中央区八丁堀2-30-16 2-30-16, Hatchobori, Chuou-ku, Tokyo 1040032, JP
Inventors:
濡木 理 NUREKI, Osamu; JP
西増 弘志 NISHIMASU, Hiroshi; JP
山田 真理 YAMADA, Mari; JP
山形 哲也 YAMAGATA, Tetsuya; US
シン ユアンバウ QIN, Yuanbo; US
Agent:
高島 一 TAKASHIMA, Hajime; JP
鎌田 光宜 KAMADA, Mitsunori; JP
Priority Data:
62/539,80401.08.2017US
Title (EN) MODIFIED Cas9 PROTEINS AND APPLICATION FOR SAME
(FR) PROTÉINES Cas9 MODIFIÉES ET APPLICATION DESDITES PROTÉINES
(JA) 改変されたCas9タンパク質及びその用途
Abstract:
(EN) Modified CjCas9 proteins that can better bind to guide RNA and are useful as tools for gene editing. For example, a protein that can bind to guide RNA and comprises a sequence that includes the amino acid sequence represented by SEQ ID NO: 2 but has arginine instead of glutamic acid at position 946, arginine instead of alanine at position 911, and arginine instead of isoleucine at position 920.
(FR) L'invention concerne des protéines CjCas9 modifiées, qui peuvent mieux se lier à l'ARN guide et qui sont avantageuses en tant qu'outils pour l'édition de gènes. Par exemple, une protéine qui peut se lier à l'ARN guide et qui comprend une séquence comportant la séquence d'acides aminés représentée par SEQ ID NO: 2, mais qui possède de l'arginine au lieu d'acide glutamique en position 946, de l'arginine au lieu d'alanine en position 911 et de l'arginine au lieu d'isoleucine en position 920, est décrite.
(JA) 配列番号2で表されるアミノ酸配列において、 946位のグルタミン酸がアルギニンに置換し、 911位のアラニンがアルギニンに置換し、 920位のイソロイシンがアルギニンに置換している アミノ酸配列を含む配列からなり、且つ、ガイドRNAとの結合能を有するタンパク質等の改変型CjCas9タンパク質は、ガイドRNAとの結合能が向上しており、遺伝子編集におけるツールとして有用である。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)