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1. (WO2019026870) NOVEL METHOD FOR MEASURING SFLT-1 (SOLUBLE VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-1)
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Pub. No.: WO/2019/026870 International Application No.: PCT/JP2018/028541
Publication Date: 07.02.2019 International Filing Date: 31.07.2018
IPC:
G01N 33/53 (2006.01) ,C12M 1/34 (2006.01) ,G01N 33/543 (2006.01) ,G01N 33/545 (2006.01) ,G01N 33/566 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
34
Measuring or testing with condition measuring or sensing means, e.g. colony counters
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
543
with an insoluble carrier for immobilising immunochemicals
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
543
with an insoluble carrier for immobilising immunochemicals
544
the carrier being organic
545
Synthetic resin
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
566
using specific carrier or receptor proteins as ligand binding reagent
Applicants:
学校法人学文館 GAKUBUNKAN [JP/JP]; 群馬県高崎市新町270-1 270-1, Shinmachi, Takasaki-shi, Gunma 3701393, JP
Inventors:
澁谷 正史 SHIBUYA Masabumi; JP
Agent:
特許業務法人平木国際特許事務所 HIRAKI & ASSOCIATES; 東京都港区愛宕二丁目5-1 愛宕グリーンヒルズMORIタワー32階 Atago Green Hills MORI Tower 32F, 5-1, Atago 2-chome, Minato-ku, Tokyo 1056232, JP
Priority Data:
2017-14928001.08.2017JP
2018-04291709.03.2018JP
Title (EN) NOVEL METHOD FOR MEASURING SFLT-1 (SOLUBLE VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-1)
(FR) NOUVEAU PROCÉDÉ DE MESURE DE SFLT-1 (RÉCEPTEUR-1 DE FACTEUR DE CROISSANCE ENDOTHÉLIALE VASCULAIRE SOLUBLE)
(JA) sFlt-1(可溶型血管内皮増殖因子受容体-1)の新規測定法
Abstract:
(EN) The purpose of the present invention is to provide an sFlt-1 measurement method for sFlt-1 protein, which is novel, simple, inexpensive and suitable for practical use. A method for measuring sFlt-1 in a sample by forming a complex in which sFlt-1 is sandwiched by heparin and an anti-sFlt-1 antibody, the method comprising: bringing heparin or an anti-sFlt-1 antibody immobilized on a support into contact with the sample to form a complex of heparin or the anti-sFlt-1 antibody and sFlt-1 in the sample on the support; and detecting the presence of the complex using a labeled anti-sFlt-1 antibody, or an anti-sFlt-1 antibody and a labeled anti-immunoglobulin antibody bonded to the labeled anti-sFlt-1 antibody, or labeled heparin.
(FR) Le but de la présente invention est de fournir un procédé de mesure de sFlt-1 pour la protéine sFlt-1 nouveau, simple, peu coûteux et permettant une utilisation pratique. L'invention concerne un procédé de mesure de sFlt-1 dans un échantillon par la formation d'un complexe dans lequel sFlt-1 est pris en sandwich par l'héparine et un anticorps anti-sFlt-1, le procédé consistant : à mettre en contact de l'héparine ou un anticorps anti-sFlt-1 immobilisé sur un support avec l'échantillon afin de former un complexe d'héparine ou de l'anticorps anti-sFlt-1 et de sFlt-1 dans l'échantillon sur le support ; et à détecter la présence du complexe à l'aide d'un anticorps anti-sFlt-1 marqué, ou d'un anticorps anti-sFlt-1 et d'un anticorps anti-immunoglobuline marqué lié à l'anticorps anti-sFlt-1 marqué, ou de l'héparine marquée.
(JA) sFlt-1タンパクに対して、新規で簡便かつ安価で実用的なsFlt-1測定法を提供すること。ヘパリンと抗sFlt-1抗体でsFlt-1を挟んだ複合体を形成させて検体中のsFlt-1を測定する方法であって、担体に固相化したヘパリンまたは抗sFlt-1抗体に検体を接触させ、該担体上にヘパリンまたは抗sFlt-1抗体と検体中のsFlt-1との複合体を形成させ、該複合体の存在を標識した抗sFlt-1抗体もしくは抗sFlt-1抗体とそれに結合する標識した抗イムノグロブリン抗体、または標識したヘパリンを用いて検出することを含む、検体中のsFlt-1を測定する方法。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)