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1. (WO2019025958) MODIFIED GENE SEQUENCES ENCODING CHOLINE OXIDASE AND A METHOD FOR PREPARING BETAINE USNG THE SAME
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Pub. No.: WO/2019/025958 International Application No.: PCT/IB2018/055712
Publication Date: 07.02.2019 International Filing Date: 31.07.2018
IPC:
C12N 9/00 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
Applicants:
ENZIBETA BIOTECH PVT. LTD. [IN/IN]; 201, Tanmayee Nivas, Lig- 32, Dharmareddy Colony, Phase 1 Kphb, Opp. Jntu, Kukatpally, Hyderabad – 500072, Telangana, India Hyderabad 500072, IN
Inventors:
GAUR, Prashant; IN
Agent:
AMBASTHA, Lalit; IN
KAUSHIK, Shruti; IN
Priority Data:
20174102708431.07.2017IN
Title (EN) MODIFIED GENE SEQUENCES ENCODING CHOLINE OXIDASE AND A METHOD FOR PREPARING BETAINE USNG THE SAME
(FR) SÉQUENCES GÉNIQUES MODIFIÉES CODANT POUR LA CHOLINE OXYDASE ET PROCÉDÉ DE PRÉPARATION DE LA BÉTAÏNE L'UTILISANT
Abstract:
(EN) The present invention provides at least two modified gene sequences, Sequence 1 comprising of nucleotide sequence of SEQ. ID no.1, and Sequence 2 comprising of nucleotide sequence of SEQ. ID no.2, encoding the enzyme choline oxidase wherein the gene sequences have been obtained by modifying the codA gene (Accession no. X84895) encoding choline oxidase from Arthrobacter globiformis, and a method to enzymatically produce betaine using choline oxidases encoded by Sequence 1, and Sequence 2, wherein the enzymatically produced betaine has minimal undesired trimethylamine contamination.
(FR) La présente invention concerne au moins deux séquences géniques modifiées, la séquence 1 comprenant une séquence nucléotidique de SEQ ID No 1, et la séquence 2 comprenant une séquence nucléotidique de SEQ ID No 2, codant pour l'enzyme choline oxydase, les séquences géniques ayant été obtenues par modification du gène codA (No d'accès X84895) codant pour la choline oxydase à partir d'Arthrobacter globiformis, et un procédé pour produire par voie enzymatique de la bétaïne à l'aide de choline oxydases codées par la séquence 1 et la séquence 2, la bétaïne produite par voie enzymatique ayant une contamination de triméthylamine indésirable minimale.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)