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1. (WO2019025597) MEANS AND METHODS FOR PROTEIN QUANTIFICATION
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Pub. No.: WO/2019/025597 International Application No.: PCT/EP2018/071152
Publication Date: 07.02.2019 International Filing Date: 03.08.2018
IPC:
G01N 33/68 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68
involving proteins, peptides or amino acids
Applicants:
FRAUNHOFER-GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E.V. [DE/DE]; Hansastrasse 27c 80686 München, DE
Inventors:
KASTILAN, Robin; DE
MÜSCHEN, Catherine; DE
BUYEL, Johannes; DE
Agent:
HERZOG FIESSER & PARTNER PATENTANWÄLTE PARTG MBB; Alexander Dick Dudenstraße 46 68167 Mannheim, DE
Priority Data:
17184690.003.08.2017EP
Title (EN) MEANS AND METHODS FOR PROTEIN QUANTIFICATION
(FR) MOYENS ET PROCÉDÉS DE QUANTIFICATION DE PROTÉINES
Abstract:
(EN) The present invention relates to biochemical test methods for protein quantification and, in particular, to the application of foam-suppressing and/or foam-destroying substances for avoiding readout problems and ensuring the accuracy of measurement results. Specifically, the present invention pertains to a method for determining the amount of one or more proteins in a solution comprising: (a) providing at least a first solution that is known to contain or suspected of containing one or more protein(s), (b) adding at least one foam-suppressing and/or foam- destroying substance to the first solution, resulting in a second solution, (c) determining the amount of the one or more protein(s) in the second solution. Encompassed by the present invention is also the use of a foam-suppressing and/or foam-destroying substance for the quantification of the protein content in a solution.
(FR) La présente invention concerne des procédés de test biochimique pour la quantification de protéines et, en particulier, l'application de substances de suppression de mousse et/ou de destruction de mousse afin d'éviter les problèmes de lecture et assurer la précision des résultats de mesure. Plus précisément, la présente invention concerne un procédé de détermination de la quantité d'une ou de plusieurs protéines dans une solution, le procédé comprenant : (a) l'obtention d'au moins une première solution qui est connue pour contenir ou suspectée de contenir une ou plusieurs protéines, (b) l'ajout d'au moins une substance de suppression de mousse et/ou de destruction de mousse à la première solution, ce qui produit une deuxième solution, (c) la détermination de la quantité desdites protéines dans la deuxième solution. La présente invention concerne également l'utilisation d'une substance de suppression de mousse et/ou de destruction de mousse pour la quantification du contenu protéique dans une solution.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)