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1. (WO2019025387) METHOD AND COMPOSITION FOR STABILIZING CELL-FREE NUCLEIC ACIDS AND CELLS
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Pub. No.: WO/2019/025387 International Application No.: PCT/EP2018/070633
Publication Date: 07.02.2019 International Filing Date: 30.07.2018
IPC:
C12Q 1/68 (2018.01) ,C12N 15/10 (2006.01) ,A61B 5/15 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
B
DIAGNOSIS; SURGERY; IDENTIFICATION
5
Measuring for diagnostic purposes; Identification of persons
15
Devices for taking samples of blood
Applicants:
SARSTEDT AG & CO. KG [DE/DE]; Sarstedtstraße 1 51582 Nümbrecht, DE
Inventors:
Agent:
TARUTTIS, Stefan; DE
Priority Data:
17184585.202.08.2017EP
Title (EN) METHOD AND COMPOSITION FOR STABILIZING CELL-FREE NUCLEIC ACIDS AND CELLS
(FR) PROCÉDÉ ET COMPOSITION POUR STABILISER DES ACIDES NUCLÉIQUES ACELLULAIRES ET CELLULES
(DE) VERFAHREN UND ZUSAMMENSETZUNG ZUR STABILISIERUNG ZELLFREIER NUKLEINSÄUREN UND ZELLEN
Abstract:
(EN) The invention relates to a composition for use as a stabilizer and a method for stabilizing biological samples, in particular of whole blood, in particular for stabilizing the content and the integrity of cell-free nucleic acids. The composition has, in aqueous solution, at least one buffer substance, which buffers to a pH value of 7 or less, preferably 3.5 to 7.0, at least one chelating agent for divalent cations and urotropine, optionally PEG, or consists thereof.
(FR) L'invention concerne une composition à utiliser en tant qu'agent de stabilisation et un procédé pour stabiliser des échantillons biologiques, notamment du sang total, notamment pour stabiliser la teneur en acides nucléiques acellulaires et leur intégrité. La composition présente, ou se compose de, au moins une substance tampon qui a une action tampon à une valeur de pH de 7 ou moins, de préférence de 3,5 à 7,0, au moins un agent de chélation pour cations bivalents et de l'urotropine, éventuellement PEG, en solution aqueuse.
(DE) Die Erfindung betrifft eine Zusammensetzung zur Verwendung als Stabilisierungsmittel und einem Verfahren zur Stabilisierung biologischer Proben, insbesondere von Vollblut, insbesondere zur Stabilisierung des Gehalts und der Integrität zellfreier Nukleinsäuren. Die Zusammensetzung weist in wässriger Lösung zumindest eine Puffersubstanz auf, die auf einen pH-Wert von 7 oder darunter, bevorzugt 3,5 bis 7,0 puffert, zumindest einen Chelatbildner für zweiwertige Kationen und Urotropin auf, optional PEG, oder besteht daraus.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: German (DE)
Filing Language: German (DE)