Mobile |
Deutsch |
Español |
Français |
日本語 |
한국어 |
Português |
Русский |
中文 |
العربية |
PATENTSCOPE
Search International and National Patent Collections
PATENTSCOPE will be unavailable a few hours for maintenance reason on Monday 18.02.2019 at 12:00 PM CET
Options
Query
Result
Interface
Office
Translate
Query Language
All
Arabic
Bulgarian
Chinese
Danish
English
Estonian
French
German
Hebrew
Indonesian
Italian
Japanese
Korean
Laotian
Polish
Portuguese
Romanian
Russian
Spanish
Swedish
Thai
Vietnamese
Stem
Sort by:
Relevance
Pub Date Desc
Pub Date Asc
App Date Desc
App Date Asc
List Length
10
50
100
200
Result List Language
Query Language
English
Spanish
Korean
Vietnamese
Hebrew
Portuguese
French
German
Japanese
Russian
Chinese
Italian
Polish
Danish
Swedish
Arabic
Estonian
Indonesian
Thai
Bulgarian
Laotian
Romanian
Displayed Fields
Application Number
Publication Date
Abstract
Applicant Name
Int. Class
Image
Inventor Name
Chart/Graph
Table
Graph
Group by
*
None
Offices of NPEs
IPC code
Applicants
Inventors
Filing Dates
Publication Dates
Countries
No of Items/Group
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
Download Fields
NPEs
Default Search Form
Simple
Advanced Search
Field Combination
Browse by Week (PCT)
Cross Lingual Expansion
Translator
Simple
Advanced Search
Field Combination
Browse by Week (PCT)
Cross Lingual Expansion
Translator
Default Tab Search Form
Front Page
Any Field
Full Text
ID/Numbers
IPC
Names
Dates
Front Page
Any Field
Full Text
ID/Numbers
IPC
Names
Dates
Interface Language
English
Deutsch
Français
Español
日本語
中文
한국어
Português
Русский
English
Deutsch
Français
Español
日本語
中文
한국어
Português
Русский
Multiple Windows Interface
Tooltip Help
IPC Tooltip Help
Instant Help
Expanded Query
Office:
All
All
PCT
Africa
African Regional Intellectual Property Organization (ARIPO)
Egypt
Kenya
Morocco
Tunisia
South Africa
Americas
United States of America
Canada
LATIPAT
Argentina
Brazil
Chile
Colombia
Costa Rica
Cuba
Dominican Rep.
Ecuador
El Salvador
Guatemala
Honduras
Mexico
Nicaragua
Panama
Peru
Uruguay
Asia-Europe
Australia
Bahrain
China
Denmark
Estonia
Eurasian Patent Office
European Patent Office (EPO)
France
Germany
Germany(DDR data)
Israel
Japan
Jordan
Portugal
Russian Federation
Russian Federation(USSR data)
Saudi Arabia
United Arab Emirates
Spain
Republic of Korea
India
United Kingdom
Georgia
Bulgaria
Italy
Romania
Lao People's Democratic Republic
Asean
Singapore
Viet Nam
Indonesia
Cambodia
Malaysia
Brunei Darussalam
Philippines
Thailand
WIPO translate (Wipo internal translation tool)
Search
Simple
Advanced Search
Field Combination
Cross Lingual Expansion
Chemical compounds (login required)
Browse
Browse by Week (PCT)
Gazette Archive
National Phase Entries
Full download
Incremental download (last 7 days)
Sequence listing
IPC Green Inventory
Portal to patent registers
Translate
WIPO Translate
WIPO Pearl
News
PATENTSCOPE News
Login
ui-button
Login
Account Sign Up
Options
Options
Help
ui-button
How to Search
User Guide PATENTSCOPE
User Guide: Cross Lingual Expansion
User Guide: ChemSearch
Query Syntax
Fields Definition
Country Code
Data Coverage
PCT applications
PCT national phase entry
National collections
Global Dossier public
FAQ
Feedback&Contact
INID codes
Kind codes
Tutorials
About
Overview
Terms And Conditions
Disclaimer
Home
IP Services
PATENTSCOPE
Machine translation
Wipo Translate
Arabic
German
English
Spanish
French
Japanese
Korean
Portuguese
Russian
Chinese
Google Translate
Bing/Microsoft Translate
Baidu Translate
Arabic
English
French
German
Spanish
Portuguese
Russian
Korean
Japanese
Chinese
...
Italian
Thai
Cantonese
Classical Chinese
Some content of this application is unavailable at the moment.
If this situation persist, please contact us at
Feedback&Contact
1. (WO2019024706) ASPARTASE VARIANT, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF
PCT Biblio. Data
Full Text
National Phase
Notices
Documents
Latest bibliographic data on file with the International Bureau
Submit observation
PermaLink
PermaLink
Bookmark
Pub. No.:
WO/2019/024706
International Application No.:
PCT/CN2018/096737
Publication Date:
07.02.2019
International Filing Date:
24.07.2018
IPC:
C12N 9/88
(2006.01) ,
C12N 15/60
(2006.01) ,
C12N 1/19
(2006.01) ,
C12P 13/04
(2006.01)
C
CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
88
Lyases (4.)
C
CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
52
Genes encoding for enzymes or proenzymes
60
Lyases (4)
C
CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
14
Fungi; Culture media therefor
16
Yeasts; Culture media therefor
19
modified by introduction of foreign genetic material
C
CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13
Preparation of nitrogen-containing organic compounds
04
Alpha- or beta-amino acids
Applicants:
秦皇岛华恒生物工程有限公司 QINHUANGDAO HUAHENG BIOENGINEERING CO., LTD.
[CN/CN]; 中国河北省秦皇岛市 山海关区沈山路18号 No. 18 Shenshan Road, Shanhaiguan District Qinhuangdao, Hebei 066200, CN
Inventors:
吴边 WU, Bian
; CN
刘洋 LIU, Yang
; CN
李瑞峰 LI, Ruifeng
; CN
张冬竹 ZHANG, Dongzhu
; CN
刘志成 LIU, Zhicheng
; CN
章晖 ZHANG, Hui
; CN
Agent:
北京纪凯知识产权代理有限公司 JEEKAI & PARTNERS
; 中国北京市 丰台区广安路9号5号楼15A层 Floor 15A, Building No. 5 9 Guang'an Road, Fengtai District Beijing 100055, CN
Priority Data:
201710659654.9
04.08.2017
CN
Title
(EN)
ASPARTASE VARIANT, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF
(FR)
VARIANTE D'ASPARTASE, SON PROCÉDÉ DE PRÉPARATION ET APPLICATION ASSOCIÉE
(ZH)
天冬氨酸酶变体及其制备方法与应用
Abstract:
(EN)
An aspartase variant, a preparation method therefor, and an application thereof are provided. The amino acid sequence of the aspartase variant has 96% or higher identity with sequence 2 in the sequence listing, comprises T187I and N326C mutations at positions 187 and 326 of sequence 2, respectively, and has improved catalytic acrylic acid amino-adding activity compared with the aspartase shown in sequence 2. Experiments prove that the aspartase variant can improve catalytic acrylic acid amino-adding activity compared with a wild-type parent aspartase, and has better thermal stability and pH spectrum, and can greatly improve the conversion of acrylic acid in acrylic acid amino-adding reactions.
(FR)
L'invention concerne une variante d'aspartase, un procédé de préparation de celle-ci, et une application de celle-ci. La séquence d'acides aminés de la variante d'aspartase présente 96 % ou plus d'identité avec la séquence 2 dans la liste de séquences, comprend des mutations T187I et N326C aux positions 187 et 326 de la séquence 2, respectivement, et présente une activité d'ajout d'acide acrylique catalytique améliorée par rapport à l'aspartase représentée en séquence 2. Des expériences prouvent que la variante d'aspartase peut améliorer l'activité d'ajout d'acide acrylique catalytique par comparaison avec une aspartase parente de type sauvage, et présente une meilleure stabilité thermique et un meilleur spectre de pH, et peut grandement améliorer la conversion de l'acide acrylique dans des réactions d'ajout d'acide acrylique.
(ZH)
提供了一种天冬氨酸酶变体及其制备方法与应用。所述天冬氨酸酶变体其氨基酸序列与序列表中序列2相比具有96%以上同一性,且在序列2的第187位和326位分别存在T187I和N326C的突变,并且与序列2所示的天冬氨酸酶相比具有改善的催化丙烯酸加氨活性。实验证明,所述天冬氨酸酶变体与野生型亲本天冬氨酸酶相比确实具有改善的催化丙烯酸加氨活性,且热稳定性和pH谱更优,在丙烯酸加氨反应中可以大大提高丙烯酸的转化率。
Designated States:
AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language:
Chinese (
ZH
)
Filing Language:
Chinese (
ZH
)