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1. (WO2019024341) METHOD FOR CONSTRUCTING LIBRARY OF CELL-FREE DNAS IN BODY FLUIDS AND APPLICATION THEREOF
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Pub. No.: WO/2019/024341 International Application No.: PCT/CN2017/113208
Publication Date: 07.02.2019 International Filing Date: 27.11.2017
IPC:
C40B 50/06 (2006.01) ,C12Q 1/68 (2018.01) ,C12N 15/10 (2006.01)
C CHEMISTRY; METALLURGY
40
COMBINATORIAL TECHNOLOGY
B
COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
50
Methods of creating libraries, e.g. combinatorial synthesis
06
Biochemical methods, e.g. using enzymes or whole viable micro-organisms
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
Applicants:
深圳华大生命科学研究院 BGI SHENZHEN [CN/CN]; 中国广东省深圳市 盐田区北山工业区综合楼 Main Building, Beishan Industrial Zone, Yantian District Shenzhen, Guangdong 518083, CN
Inventors:
王明月 WANG, Mingyue; CN
刘龙奇 LIU, Longqi; CN
刘传宇 LIU, Chuanyu; CN
吴亮 WU, Liang; CN
许江山 XU, Jiangshan; CN
袁月 YUAN, Yue; CN
商周春 SHANG, Zhouchun; CN
Agent:
北京品源专利代理有限公司 BEYOND ATTORNEYS AT LAW; 中国北京市 海淀区莲花池东路39号西金大厦6层 F6, Xijin Centre 39 Lianhuachi East Rd., Haidian District Beijing 100036, CN
Priority Data:
Title (EN) METHOD FOR CONSTRUCTING LIBRARY OF CELL-FREE DNAS IN BODY FLUIDS AND APPLICATION THEREOF
(FR) PROCÉDÉ DE CONSTRUCTION D'UNE BIBLIOTHÈQUE D'ADN ACELLULAIRE DANS DES LIQUIDES CORPORELS ET APPLICATION ASSOCIÉE
(ZH) 一种体液游离DNA的文库构建方法及其应用
Abstract:
(EN) A method for constructing a library of cell-free DNAs in body fluids, comprising directly acting a transposase or an endonuclease on a body fluid sample, fragmenting the cell-free DNAs within, and performing amplification to obtain a library. Also provided is a test kit using the present method for prenatal diagnosis or early detection of cancer.
(FR) L'invention concerne un procédé de construction d'une bibliothèque d'ADN acellulaire dans des liquides corporels, consistant à faire agir directement une transposase ou une endonucléase sur un échantillon de liquide corporel, à fragmenter l’ADN acellulaire, et à effectuer une amplification pour obtenir une bibliothèque. L'invention concerne également une trousse d'analyse utilisant le présent procédé pour le diagnostic prénatal ou la détection précoce du cancer.
(ZH) 一种对体液样本游离DNA进行文库构建的方法,包括利用转座酶或内切酶直接作用于体液样本,将其中游离DNA片段化,然后进行扩增获得文库。还提供了利用该方法进行产前诊断或癌症早期发现的试剂盒。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)