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1. (WO2019024309) GENETIC ENGINEERING STRAIN
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Pub. No.: WO/2019/024309 International Application No.: PCT/CN2017/110338
Publication Date: 07.02.2019 International Filing Date: 10.11.2017
IPC:
C12N 1/21 (2006.01) ,C12P 7/22 (2006.01) ,C12P 7/42 (2006.01) ,C12R 1/19 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
20
Bacteria; Culture media therefor
21
modified by introduction of foreign genetic material
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7
Preparation of oxygen-containing organic compounds
02
containing a hydroxy group
22
aromatic
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7
Preparation of oxygen-containing organic compounds
40
containing a carboxyl group
42
Hydroxy carboxylic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
R
INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1
Micro-organisms
01
Bacteria or actinomycetales
185
Escherichia
19
Escherichia coli
Applicants:
江南大学 JIANGNAN UNIVERSITY [CN/CN]; 中国江苏省无锡市 蠡湖大道1800号耿晓岳 Xiaoyue Geng No. 1800 Lake Avenue Wuxi, Jiangsu 214122, CN
Inventors:
蔡宇杰 CAI, Yujie; CN
刘金彬 LIU, Jinbin; CN
熊天真 XIONG, Tianzhen; CN
丁彦蕊 DING, Yanrui; CN
白亚军 BAI, Yajun; CN
郑晓晖 ZHENG, Xiaohui; CN
Agent:
哈尔滨市阳光惠远知识产权代理有限公司 HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO.,LTD.; 中国黑龙江省哈尔滨市 松北区科技一街99号18栋E501耿晓岳 Xiaoyue Geng Room E501, Building 18, Science and technology 1st street No.99, District Songbei Harbin, Heilongjiang 150028, CN
Priority Data:
201710659225.104.08.2017CN
Title (EN) GENETIC ENGINEERING STRAIN
(FR) SOUCHE GÉNÉTIQUEMENT MODIFIÉE
(ZH) 一种基因工程菌
Abstract:
(EN) A recombinant Escherichia coli (E. coli) genetically engineered strain, a construction method therefor and application thereof in biosynthesis of phenylethanol compounds, wherein L-amino acid oxidase gene, alpha-keto acid decarboxylase gene, alcohol dehydrogenase gene and enzyme gene capable of reducing NAD(P) to NAD(P)H are introduced into the genetically engineered strain.
(FR) L'invention concerne une souche recombinante d'Escherichia coli (E. coli) génétiquement modifiée, sa méthode de construction et son application dans la biosynthèse de composés phényléthanol, un gène de la L-aminoacide oxydase, un gène de l'alpha-céto-acide décarboxylase, un gène de l'alcool déshydrogénase et un gène d'une enzyme capable de réduire le NAD(P) en NAD(P)H étant introduits dans la souche génétiquement modifiée.
(ZH) 一种重组大肠杆菌基因工程菌及其构建方法和在生物合成苯乙醇类化合物中的应用,所述基因工程菌中导入了L-氨基酸氧化酶基因、α-酮酸脱羧酶基因、醇脱氢酶基因和可将NAD(P)还原成NAD(P)H的酶基因。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)