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1. (WO2019024148) COLLOIDAL GOLD CHROMATOGRAPHY RAPID DIAGNOSTIC TEST STRIP FOR DETECTING ACETYLCHOLINESTERASE AND PREPARATION METHOD THEREFOR
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Pub. No.: WO/2019/024148 International Application No.: PCT/CN2017/098611
Publication Date: 07.02.2019 International Filing Date: 23.08.2017
IPC:
G01N 33/558 (2006.01) ,G01N 33/577 (2006.01) ,G01N 33/531 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
558
using diffusion or migration of antigen or antibody
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
577
involving monoclonal antibodies
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
531
Production of immunochemical test materials
Applicants:
南京医科大学第一附属医院 THE FIRST AFFILIATED HOSPITAL OF NANJING MEDICAL UNIVERSITY [CN/CN]; 中国江苏省南京市 鼓楼区广州路300号 No. 300, Guangzhou Road, Gulou District Nanjing, Jiangsu 210029, CN
Inventors:
眭涛 SUI, Tao; CN
曹晓建 CAO, Xiaojian; CN
戈应滨 GE, Yingbin; CN
唐健 TANG, Jian; CN
葛大伟 GE, Dawei; CN
Agent:
南京知识律师事务所 NANJING ZHISHI LAW FIRM OF INTELLECTUAL PROPERTY; 中国江苏省南京市 新模范马路5号南京科技广场B座9楼 9F, Block B Nanjing Sci-Tech Center, 5# Xinmofan Road Nanjing, Jiangsu 210009, CN
Priority Data:
201710641207.031.07.2017CN
Title (EN) COLLOIDAL GOLD CHROMATOGRAPHY RAPID DIAGNOSTIC TEST STRIP FOR DETECTING ACETYLCHOLINESTERASE AND PREPARATION METHOD THEREFOR
(FR) BANDELETTE DE TEST DE DIAGNOSTIC RAPIDE PAR CHROMATOGRAPHIE D'OR COLLOÏDAL PERMETTANT LA DÉTECTION DE L'ACÉTYLCHOLINESTÉRASE ET PROCÉDÉ DE PRÉPARATION CORRESPONDANT
(ZH) 用于检测乙酰胆碱酯酶的胶体金层析快速诊断试纸条及其制备方法
Abstract:
(EN) A colloidal gold chromatography rapid diagnostic test strip for detecting acetylcholinesterase and a preparation method therefor. The diagnostic test strip comprises water-absorbing filter paper sequentially connected on a hard plastic bottom plate, a detection pad comprising a cellulose acetate membrane on which an anti-acetylcholinesterase monoclonal antibody (detection zone) and a rabbit anti-mouse IgG (quality control zone) are immobilized, a colloidal gold pad labeled with an anti-acetylcholinesterase monoclonal antibody, and a sample pad for placing a sample. The colloidal gold test strip is used for visual inspection of acetylcholinesterase in solutions, and has the following advantages: 1) extremely small amounts of reagents and samples are used, and the amount of sample can be as low as 10-20 μl, thus increasing the concentration of acetylcholinesterase in sample eluates, reducing false negative results; 2) no expensive instruments are required, thus being more suitable for field application; 3) time is greatly shortened, and detection speed is increased; and 4) the test strip can be stored at room temperature, with the only need being to keep the test strip dry.
(FR) L'invention concerne une bandelette de test de diagnostic rapide par chromatographie d'or colloïdal permettant la détection de l'acétylcholinestérase et un procédé de préparation correspondant. La bandelette de test de diagnostic comprend du papier filtre absorbant l'eau relié de manière séquentielle sur une plaque inférieure en plastique dur, un tampon de détection comprenant une membrane d'acétate de cellulose sur laquelle un anticorps monoclonal anti-acétylcholinestérase (zone de détection) et une IgG anti-souris de lapin (zone de contrôle de qualité) sont immobilisées, un tampon d'or colloïdal marqué avec un anticorps monoclonal anti-acétylcholinestérase, et un tampon d'échantillon permettant de placer un échantillon. La bande de test d'or colloïdal est utilisée pour l'inspection visuelle de l'acétylcholinestérase dans des solutions et présente les avantages suivants : 1) des quantités extrêmement faibles de réactifs et d'échantillons sont utilisées, la quantité d'échantillon pouvant aller jusqu'à 10-20 µl, ce qui permet d'augmenter la concentration d'acétylcholinestérase dans des éluats d'échantillon et de réduire les résultats faux-négatifs ; 2) aucun instrument onéreux n'est nécessaire, ce qui est plus avantageux pour une application de terrain ; 3) la durée est considérablement raccourcie et la vitesse de détection est augmentée ; et 4) la bandelette de test peut être stockée à température ambiante, la seule exigence étant le maintien de la bandelette de test dans un état sec.
(ZH) 一种用于检测乙酰胆碱酯酶的胶体金层析快速诊断试纸条及其制备方法,所述诊断试纸条包括设在硬质塑料底板上依次相连的吸水滤纸、固定有抗乙酰胆碱酯酶单克隆抗体(检测区)与兔抗小鼠IgG(质控区)的醋酸纤维膜的检测垫、标记有抗乙酰胆碱酯酶单克隆抗体的胶体金垫和放置样品的样品垫。胶体金试纸条用于肉眼检测溶液中的乙酰胆碱酯酶,具有以下优点:①试剂和样本用量极小,样本量可低至10~20μl,因而可以增加标本洗脱液中乙酰胆碱酯酶的浓度,不易出现假阴性结果;②不需贵重仪器,更适于现场应用;③时间大大缩短,提高了检测速度;④试纸条可在室温保存,只需保持试纸条干燥即可。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)