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1. (WO2019024081) TALE RVD SPECIFICALLY RECOGNIZING DNA BASE MODIFIED BY METHYLATION AND APPLICATION THEREOF
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Pub. No.: WO/2019/024081 International Application No.: PCT/CN2017/095988
Publication Date: 07.02.2019 International Filing Date: 04.08.2017
IPC:
C07K 14/195 (2006.01) ,C07K 19/00 (2006.01) ,C12N 15/31 (2006.01) ,C12N 15/63 (2006.01) ,C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195
from bacteria
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
19
Hybrid peptides
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
31
Genes encoding microbial proteins, e.g. enterotoxins
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
北京大学 PEKING UNIVERSITY [CN/CN]; 中国北京市 海淀区颐和园路5号 No.5 Yiheyuan Road,Haidian District, Beijing 100871, CN
博雅辑因(北京)生物科技有限公司 EDIGENE INC. [CN/CN]; 中国北京市 昌平区科学园路22号2栋2层(中关村生命科学园内) Life Science Park, Floor 2, Building 2,22 KeXueYuan Road, Changping District, Beijing 102206, CN
Inventors:
魏文胜 WEI, Wensheng; CN
伊成器 YI, Chengqi; CN
张媛 ZHANG, Yuan; CN
郭生杰 GUO, Shengjie; CN
朱晨旭 ZHU, Chenxu; CN
刘璐璐 LIU, Lulu; CN
Agent:
北京知元同创知识产权代理事务所(普通合伙) BEIJING ORIGINTELLIGENCE IP LAW FIRM; 中国北京市 海淀区上地三街9号嘉华大厦E座705室刘元霞 LIU Yuanxia Room 705, Tower E, Jiahua Building No.9, Shangdi 3rd Street, Haidian District, Beijing 100085, CN
Priority Data:
Title (EN) TALE RVD SPECIFICALLY RECOGNIZING DNA BASE MODIFIED BY METHYLATION AND APPLICATION THEREOF
(FR) RVD DE TALE RECONNAISSANT SPÉCIFIQUEMENT UNE BASE D'ADN MODIFIÉE PAR MÉTHYLATION ET APPLICATION CORRESPONDANTE
(ZH) 特异性识别甲基化修饰DNA碱基的TALE RVD及其应用
Abstract:
(EN) The present invention provides an RVD having recognition preference for and different binding properties to 5mC, 5hmC, and 6mA, an isolated DNA-binding polypeptide comprising a TALE repeat domain containing the RVD, a fusion protein, a polynucleotide encoding the same, a vector comprising the polynucleotide, and a host cell. The present invention also provides applications of the DNA-binding polypeptide and fusion protein in methylation-dependent gene activation, efficient genome editing, detection of a methylated base in a target sequence of a target gene, or targeted binding to a target gene in a cell.
(FR) La présente invention concerne un RVD présentant une préférence de reconnaissance pour et différentes propriétés de liaison à 5mC, 5hmC et 6mA, un polypeptide de liaison à l'ADN isolé comprenant un domaine de répétition TALE contenant le RVD, une protéine de fusion, un polynucléotide codant pour celle-ci, un vecteur comprenant le polynucléotide et une cellule hôte. La présente invention concerne également des applications du polypeptide de liaison à l'ADN et de la protéine de fusion dans l'activation génique dépendant de la méthylation, une édition de génome efficace, la détection d'une base méthylée dans une séquence cible d'un gène cible ou la liaison ciblée à un gène cible dans une cellule.
(ZH) 本发明提供了对于5mC、5hmC和6mA具有识别偏好和不同结合特性的RVD,以及包含含有上述RVD的TALE重复结构域的分离的DNA结合多肽、融合蛋白、其编码多核苷酸、包含多核苷酸的载体以及宿主细胞。本发明还提供上述DNA结合多肽、融合蛋白在甲基化依赖的基因激活、高效基因组编辑、检测目标基因靶序列中的甲基化碱基以及靶向结合细胞中目标基因中的应用。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)