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1. (WO2019023937) HIGHLY EFFICIENT ENDONUCLEASE BUFFER SYSTEM
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Pub. No.: WO/2019/023937 International Application No.: PCT/CN2017/095470
Publication Date: 07.02.2019 International Filing Date: 01.08.2017
IPC:
C12N 9/96 (2006.01) ,C12N 9/22 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
96
Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
16
acting on ester bonds (3.1)
22
Ribonucleases
Applicants:
深圳华大智造科技有限公司 MGI TECH CO., LTD. [CN/CN]; 中国广东省深圳市 盐田区北山工业区综合楼及11栋2楼 Main Building and Second Floor of No.11 Building Beishan Industrial Zone, Yantian District Shenzhen, Guangdong 518083, CN
Inventors:
张长领 ZHANG, Changling; CN
刘二凯 LIU, Erkai; CN
陈奥 CHEN, Ao; CN
章文蔚 ZHANG, Wenwei; CN
Agent:
深圳鼎合诚知识产权代理有限公司 DHC IP ATTORNEYS; 中国广东省深圳市 福田区金田路与福华路交汇处现代商务大厦2201 Suite 2201, Modern International Commercial Building, Cross of Fuhua Road and Jintian Road, Futian District Shenzhen, Guangdong 518048, CN
Priority Data:
Title (EN) HIGHLY EFFICIENT ENDONUCLEASE BUFFER SYSTEM
(FR) SYSTÈME TAMPON D'ENDONUCLÉASE EXTRÊMEMENT EFFICACE
(ZH) 一种高效的内切酶缓冲液体系
Abstract:
(EN) Disclosed is an endonuclease buffer capable of increasing Endo IV enzyme activity, the buffer comprising 20 to 200 mM Tris-HCl, 0.05 to 0.2 M NaCl, 2 to 20 mM MgSO4, 0.2 to 10 mM DTT and 0.05 to 5 mass% of a surfactant, wherein the pH is 7 to 8. The buffer may further comprise 0.1 to 5 mass% of short-chain alcohols having fewer than five carbon atoms.
(FR) L'invention concerne un tampon d'endonucléase capable d'augmenter l'activité enzymatique d'Endo IV, le tampon comprenant de 20 à 200 mM de Tris-HCl, de 0,05 à 0,2 M de NaCl, de 2 à 20 mM de MgSO4, de 0,2 à 10 mM de DTT et de 0,05 à 5 % en masse d'un tensioactif, le pH étant de 7 à 8. Le tampon peut en outre comprendre de 0,1 à 5 % en masse d'alcools à chaîne courte ayant moins de cinq atomes de carbone.
(ZH) 一种能提高Endo IV酶活性的内切酶缓冲液,包括20~200mM的Tris-HCl,0.05~0.2M的NaCl,2~20mM的MgSO 4,0.2~10mM的DTT,0.05~5质量%的表面活性剂,pH为7~8。所述缓冲液还可进一步包括0.1~5质量%的碳原子数小于5的短链醇类。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)