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1. (WO2019023924) METHODS OF ENRICHING AND DETERMINING TARGET NUCLEOTIDE SEQUENCES
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Pub. No.: WO/2019/023924 International Application No.: PCT/CN2017/095404
Publication Date: 07.02.2019 International Filing Date: 01.08.2017
IPC:
C12Q 1/68 (2018.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
深圳恒特基因有限公司 HELITEC LIMITED [CN/CN]; 中国广东省深圳市 南山区西丽街道中山园路1001号TCL国际E城F3栋6A Room 6A, Building F3, TCL International E-City, 1001 Zhongshanyuan Road, Xili Street, Nanshan District Shenzhen, Guangdong 518000, CN
Inventors:
郑宗立 ZHENG, Zongli; CN
Agent:
广州三环专利商标代理有限公司 SCIHEAD IP LAW FIRM; 中国广东省广州市 越秀区先烈中路80号汇华商贸大厦1508室 Room 1508, Huihua Commercial & Trade Building, No. 80 Xian Lie Zhong Road, Yuexiu District Guangzhou, Guangdong 510070, CN
Priority Data:
Title (EN) METHODS OF ENRICHING AND DETERMINING TARGET NUCLEOTIDE SEQUENCES
(FR) PROCÉDÉS D'ENRICHISSEMENT ET DE DÉTERMINATION DE SÉQUENCES NUCLÉOTIDIQUES CIBLES
Abstract:
(EN) The present invention provides methods, compositions and kits for enriching and determining nucleotide sequences of a plurality of target loci from a sample comprising nucleic acids. The methods comprise one or more cycles of primer extension followedby PCR amplification of target sequences using nested target-specific primers.
(FR) La présente invention concerne des procédés, des compositions et des kits pour enrichir et déterminer des séquences nucléotidiques d'une pluralité de loci cibles à partir d'un échantillon comprenant des acides nucléiques. Les procédés comprennent un ou plusieurs cycles d'extension d'amorce suivis par une amplification par PCR de séquences cibles à l'aide d'amorces incluses spécifiques d'une cible.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)