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1. (WO2019009364) PLATELET PRODUCTION METHOD AND APPARATUS AND METHOD FOR DETERMINING OPERATING CONDITIONS IN PLATELET PRODUCTION APPARATUS
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Pub. No.: WO/2019/009364 International Application No.: PCT/JP2018/025537
Publication Date: 10.01.2019 International Filing Date: 05.07.2018
IPC:
C12N 5/078 (2010.01) ,B01F 7/16 (2006.01) ,B01F 7/18 (2006.01) ,B01F 11/00 (2006.01) ,C12M 1/02 (2006.01) ,C12M 3/00 (2006.01) ,C12N 5/10 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
078
Cells from blood or from the immune system
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
F
MIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
7
Mixers with rotary stirring devices in fixed receptacles; Kneaders
16
with stirrers rotating about a vertical axis
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
F
MIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
7
Mixers with rotary stirring devices in fixed receptacles; Kneaders
16
with stirrers rotating about a vertical axis
18
with paddles or arms
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
F
MIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
11
Mixers with shaking, oscillating, or vibrating mechanisms
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
02
with agitation means; with heat exchange means
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3
Tissue, human, animal or plant cell, or virus culture apparatus
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10
Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
Applicants:
国立大学法人京都大学 KYOTO UNIVERSITY [JP/JP]; 京都府京都市左京区吉田本町36番地1 36-1, Yoshida-honmachi, Sakyo-ku, Kyoto-shi, Kyoto 6068501, JP
株式会社メガカリオン MEGAKARYON CORPORATION [JP/JP]; 京都府京都市下京区中堂寺栗田町93番地 京都リサーチパーク Kyoto Research Park, 93 Awatacho, Chudoji, Shimogyo-ku, Kyoto-shi, Kyoto 6008815, JP
佐竹化学機械工業株式会社 SATAKE CHEMICAL EQUIPMENT MFG.,LTD. [JP/JP]; 埼玉県戸田市新曽66番地 66, Niizo, Toda-shi, Saitama 3350021, JP
Inventors:
江藤 浩之 ETO, Koji; JP
中村 壮 NAKAMURA, Sou; JP
伊東 幸敬 ITO, Yukitaka; JP
重盛 智大 SHIGEMORI, Tomohiro; JP
加藤 好一 KATO, Yoshikazu; JP
Agent:
奥山 尚一 OKUYAMA, Shoichi; JP
有原 幸一 ARIHARA, Koichi; JP
松島 鉄男 MATSUSHIMA, Tetsuo; JP
中村 綾子 NAKAMURA, Ayako; JP
Priority Data:
2017-13359207.07.2017JP
Title (EN) PLATELET PRODUCTION METHOD AND APPARATUS AND METHOD FOR DETERMINING OPERATING CONDITIONS IN PLATELET PRODUCTION APPARATUS
(FR) PROCÉDÉ ET APPAREIL DE PRODUCTION DE PLAQUETTES ET PROCÉDÉ DE DÉTERMINATION DE CONDITIONS DE FONCTIONNEMENT DANS UN APPAREIL DE PRODUCTION DE PLAQUETTES
(JA) 血小板の製造方法および製造装置、ならびに血小板の製造装置における運転条件の決定方法
Abstract:
(EN) Provided are a method and an apparatus for stably producing sound and functional platelets and a method for determining operating conditions in the platelet production apparatus. The present invention relates to a production method including a step for culturing megakaryocyte cells in a platelet production medium C, wherein the culturing step includes a step for stirring the medium C in containers 1, 4 by using stirring mechanisms 2, 5. In one production method, the stirring step involves reciprocating agitating blades 21, 24 of the stirring mechanism 2 so as to generate a predetermined turbulent flow energy, a shear stress, or a Kolmogorov scale in the medium C. In another production method, the stirring step involves rotating a stirring blade 51 of the stirring mechanism 5 in a state in which a plurality of stationary members 53 is arranged therearound in a bottom region 4e of the container 4 so that a turbulent flow is generated in the medium C. The present invention also relates to a production apparatus for carrying out the production method. The present invention also relates to a method for determining operating conditions in the platelet production apparatus.
(FR) La présente invention concerne un procédé et un appareil pour produire de manière stable des plaquettes saines et fonctionnelles et un procédé de détermination de conditions de fonctionnement dans l'appareil de production de plaquettes. La présente invention concerne un procédé de production comprenant une étape de culture de cellules mégacaryocytaires dans un milieu de production de plaquettes C, l'étape de culture comprenant une étape d'agitation du milieu C dans des récipients 1, 4 à l'aide de mécanismes d'agitation 2, 5. Dans un procédé de production, l'étape d'agitation comprend l’alternance de pales d'agitation 21, 24 du mécanisme d'agitation 2 de façon à générer une énergie d'écoulement turbulent prédéterminée, une contrainte de cisaillement, ou un gradient de Kolmogorov dans le milieu C. Dans un autre procédé de production, l'étape d'agitation comprend la rotation d'une pale d'agitation 51 du mécanisme d'agitation 5 dans un état dans lequel une pluralité d'éléments stationnaires 53 est disposée autour de celui-ci dans une région inférieure 4e du récipient 4 de telle sorte qu'un écoulement turbulent soit généré dans le milieu C. La présente invention concerne également un appareil de production pour la mise en œuvre du procédé de production. La présente invention concerne également un procédé de détermination de conditions de fonctionnement dans l’appareil de production de plaquettes.
(JA) 健康で機能的な血小板を安定的に産生する製造方法および製造装置、ならびに血小板の製造装置における運転条件の決定方法を提供する。本発明は、血小板産生培地中Cの巨核球細胞を培養する工程を含み、培養する工程が、撹拌機構2,5を用いて容器1,4内の培地Cを撹拌する工程を含む、製造方法に関する。1つの製造方法では、撹拌する工程が、培地C中にて所定の乱流エネルギー、剪断応力、またはコルモゴロフスケールのいずれかを発生させるように撹拌機構2の撹拌翼21,24を往復動させる。別の製造方法では、撹拌する工程が、培地Cに乱流を発生させるように、容器4の底側領域4e内にて、撹拌機構5の撹拌翼51をその周囲に複数の静止部材53を配置した状態で旋回させることを含む。本発明はまた、当該製造方法を実施する製造装置に関する。本発明はまた、血小板の製造装置における運転条件の決定方法に関する。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)