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1. (WO2019006390) AAV VECTOR COLUMN PURIFICATION METHODS
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Pub. No.: WO/2019/006390 International Application No.: PCT/US2018/040430
Publication Date: 03.01.2019 International Filing Date: 29.06.2018
IPC:
B01D 15/34 (2006.01) ,B01D 15/36 (2006.01) ,B01D 61/38 (2006.01) ,C12N 15/864 (2006.01)
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
D
SEPARATION
15
Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
08
Selective adsorption, e.g. chromatography
26
characterised by the separation mechanism
34
Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
D
SEPARATION
15
Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
08
Selective adsorption, e.g. chromatography
26
characterised by the separation mechanism
36
involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
D
SEPARATION
61
Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
38
Liquid-membrane separation
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
86
Viral vectors
864
Parvoviral vectors
Applicants:
SPARK THERAPEUTICS, INC. [US/US]; 3737 Market Street, Suite 1300 Philadelphia, Pennsylvania 19104, US
Inventors:
OH, Younghoon; US
QU, Guang; US
Agent:
BEDGOOD, Robert M.; US
Priority Data:
62/527,63330.06.2017US
62/531,74412.07.2017US
62/567,90504.10.2017US
Title (EN) AAV VECTOR COLUMN PURIFICATION METHODS
(FR) MÉTHODE DE PURIFICATION DE COLONNE DE VECTEUR AAV
Abstract:
(EN) Described and provided herein are purification, production and manufacturing methods for recombinant adeno-associated viral (rAAV) vector particles. Purification, production and manufacturing methods set forth herein, for example, include at least 2 column chromatography steps. Column chromatography steps include, for example, cation exchange chromatography, anion exchange chromatography, size exclusion chromatography and/or AAV affinity chromatography alone or in combination and in any order.
(FR) L'invention concerne des méthodes de purification, de production et de fabrication de particules recombinantes de vecteur viral adéno-associé (rAAV). Les méthodes de purification, de production et de fabrication selon l'invention font par exemple appel à au moins 2 étapes de chromatographie en colonne. Les étapes de chromatographie en colonne comprennent, par exemple, la chromatographie par échange de cations, la chromatographie par échange d'anions, la chromatographie par exclusion de taille et/ou la chromatographie par affinité d'AAV seule ou en combinaison et dans n'importe quel ordre.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)