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1. WO2019005973 - SYNTHETASE VARIANTS FOR INCORPORATION OF BIPHENYLALANINE INTO A PEPTIDE

Publication Number WO/2019/005973
Publication Date 03.01.2019
International Application No. PCT/US2018/039764
International Filing Date 27.06.2018
IPC
C12P 21/02 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
02having a known sequence of two or more amino acids, e.g. glutathione
C12N 9/10 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10Transferases (2.)
CPC
C12N 9/104
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
10Transferases (2.)
1025Acyltransferases (2.3)
104Aminoacyltransferases (2.3.2)
C12Y 601/0102
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
YENZYMES
601Ligases forming carbon-oxygen bonds (6.1)
01Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
0102Phenylalanine-tRNA ligase (6.1.1.20)
Applicants
  • PRESIDENT AND FELLOWS OF HARVARD COLLEGE [US]/[US]
Inventors
  • KUNJAPUR, Aditya Mohan
  • CHURCH, George M.
  • STORK, Devon Alexander Olson
  • KURU, Erkin
Agents
  • IWANICKI, John P.
Priority Data
62/527,11530.06.2017US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) SYNTHETASE VARIANTS FOR INCORPORATION OF BIPHENYLALANINE INTO A PEPTIDE
(FR) VARIANTS DE SYNTHÉTASE POUR INCORPORATION DE BIPHÉNYLALANINE DANS UN PEPTIDE
Abstract
(EN) This disclosure provides variants of the biphenylalanine (BipA) orthogonal translation system used for incorporation of BipA into proteins. Specifically, engineered BipA aminoacyl-tRNA synthetase (BipARS) variants and tRNA variants that improve selectivity towards BipA are described. Furthermore, this disclosure provides methods used to generate these variants.
(FR) La présente invention concerne des variants du système de translation orthogonale de biphénylalanine (BipA) utilisé pour l'incorporation de BipA dans des protéines. De manière spécifique, l'invention concerne des variants de BipA aminoacyl-ARNt synthétase (BipARS) modifiés et des variants d'ARNt qui améliorent la sélectivité vis-à-vis de BipA. En outre, la présente invention concerne des procédés utilisés pour générer ces variants.
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