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1. (WO2019005806) BODILY FLUID TARGET ENRICHMENT
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Pub. No.: WO/2019/005806 International Application No.: PCT/US2018/039518
Publication Date: 03.01.2019 International Filing Date: 26.06.2018
IPC:
C12P 19/34 (2006.01) ,C12Q 1/68 (2018.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19
Preparation of compounds containing saccharide radicals
26
Preparation of nitrogen-containing carbohydrates
28
N-glycosides
30
Nucleotides
34
Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
GENETICS RESEARCH, LLC, D/B/A ZS GENETICS, INC. [US/US]; 9 Audubon Road Wackefield, MA 01880, US
Inventors:
SHUBER, Anthony, P.; US
Agent:
MEYERS, Thomas, C.; US
HYDE, Zachary, D.; US
KOCK, Annie, J.; US
LEONARDO, Mark, S.; US
MAGGIN, Benjamin, D.; US
Priority Data:
62/526,09128.06.2017US
62/672,21716.05.2018US
Title (EN) BODILY FLUID TARGET ENRICHMENT
(FR) ENRICHISSEMENT DE CIBLE DE FLUIDE CORPOREL
Abstract:
(EN) The invention provides methods for capturing target nucleic acid directly from bodily fluid samples, without the need for certain complex sample preparation steps, using Cas endonuclease to bind to the target nucleic acid sequences. The Cas proteins, along with their sequence-specific guide RNAs, may be introduced directly into the sample, where the Cas proteins bind to ends of a target nucleic acid. The target nucleic acid is thus isolated or enriched in a sequence-specific manner. The target nucleic acid may then be subject to any suitable detection or analysis assay, such as amplification or sequencing. The target nucleic acid may be enriched by digesting other, unbound nucleic acids present in the sample with exonuclease. The bound Cas proteins prevent exonuclease from digesting the target nucleic acid, thereby leaving the only the target nucleic acid substantially present in the sample.
(FR) L'invention concerne des méthodes de capture d'acide nucléique cible directement à partir d'échantillons de fluide corporel, sans avoir besoin de certaines étapes de préparation d'échantillon complexes, à l'aide d'endonucléase Cas pour se lier aux séquences d'acides nucléiques cibles. Les protéines Cas, conjointement avec leurs ARN guides spécifiques à une séquence, peuvent être introduites directement dans l'échantillon, les protéines Cas se liant aux extrémités d'un acide nucléique cible. L'acide nucléique cible est ainsi isolé ou enrichi d'une manière spécifique à une séquence. L'acide nucléique cible peut ensuite être soumis à tout dosage approprié de détection ou d'analyse, tel que l'amplification ou le séquençage. L'acide nucléique cible peut être enrichi par digestion d'autres acides nucléiques non liés présents dans l'échantillon avec une exonucléase. Les protéines Cas liées empêchent l'exonucléase de digérer l'acide nucléique cible, ce qui ne laisse ainsi que l'acide nucléique cible sensiblement présent dans l'échantillon.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)