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1. (WO2019004556) VERO CELL LINE SUSPENSION-CULTURED IN SERUM-FREE MEDIUM AND METHOD FOR PRODUCING VACCINE VIRUS USING SAME
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Pub. No.: WO/2019/004556 International Application No.: PCT/KR2018/001905
Publication Date: 03.01.2019 International Filing Date: 13.02.2018
IPC:
C12N 5/071 (2010.01) ,C12N 7/00 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7
Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
Applicants:
에스케이바이오사이언스 주식회사 SK BIOSCIENCE CO., LTD. [KR/KR]; 경기도 성남시 분당구 판교로 310 310, Pangyo-ro, Bundang-gu Seongnam-Si Gyeonggi-do 13494, KR
Inventors:
곽준석 KWAK, Jun-seok; KR
함동수 HAM, Dong Soo; KR
이건세 LEE, Kun Se; KR
이수진 LEE, Su jeen; KR
김창신 KIM, Chang Shin; KR
김훈 KIM, Hun; KR
이현주 LEE, Hyun-joo; KR
Agent:
특허법인 필앤온지 PHIL & ONZI INT'L PATENT & LAW FIRM; 서울시 서초구 서초중앙로 36, 3층 3F., 36, Seochojungang-ro, Seocho-gu, Seoul 06643, KR
Priority Data:
10-2017-008053126.06.2017KR
Title (EN) VERO CELL LINE SUSPENSION-CULTURED IN SERUM-FREE MEDIUM AND METHOD FOR PRODUCING VACCINE VIRUS USING SAME
(FR) SUSPENSION DE LIGNÉE DE CELLULES VÉRO CULTIVÉE DANS UN MILIEU SANS SÉRUM ET PROCÉDÉ DE PRODUCTION DE VIRUS VACCINAL UTILISANT LADITE SUSPENSION
(KO) 무혈청 배지에서 부유배양 되는 VERO 세포주 및 상기 세포를 사용하여 백신용 바이러스를 제조하는 방법
Abstract:
(EN) The present invention relates to a Vero cell line which is derived from the Vero cell deposited as the ATCC CCL-81 cell line, may be cultured without serum and may be suspension cultured, and preferably, to the cell line Vero Sky7458 having a low or no tumorigenicity. In addition, the present invention relates to a culture method for growing the Vero cell and a method for producing a vaccine virus using the Vero cell.
(FR) La présente invention concerne une lignée de cellules Vero qui est dérivée de la cellule Vero déposée en tant que lignée cellulaire ATCC CCL-81, pouvant être cultivée sans sérum et pouvant être cultivée en suspension, et de préférence, la lignée cellulaire Vero Sky7458 présentant une tumorigénicité faible ou nulle. De plus, la présente invention concerne un procédé de culture destiné à la culture de la cellule Vero et un procédé de production d'un virus vaccinal à l'aide de la cellule Vero.
(KO) 본 발명은 ATCC CCL-81 세포주로서 기탁된 Vero 세포로부터 유도되고, 혈청 없이 배양이 가능하며, 부유 배양이 가능한 Vero 세포주, 바람직하게는 종양형성능이 낮거나 없는 것을 특징으로 하는 세포주 Vero Sky7458 에 관한 것이다. 또한, 본 발명은 상기 Vero 세포를 성장시키는 배양 방법 및 상기 Vero 세포를 이용하여 백신용 바이러스를 제조하는 방법에 관한 것이다.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Korean (KO)
Filing Language: Korean (KO)