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1. (WO2019004361) METHOD FOR PRODUCING LAYERED CELL SHEET AND LAYERED CELL SHEET PRODUCED BY THE SAME
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2019/004361 International Application No.: PCT/JP2018/024605
Publication Date: 03.01.2019 International Filing Date: 28.06.2018
IPC:
C12N 5/077 (2010.01) ,C12N 5/00 (2006.01) ,A61L 27/38 (2006.01) ,A61K 35/34 (2015.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
077
Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
L
METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICAL ARTICLES
27
Materials for prostheses or for coating prostheses
36
containing ingredients of undetermined constitution or reaction products thereof
38
Animal cells
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35
Medicinal preparations containing material or reaction products thereof with undetermined constitution
12
Materials from mammals or birds
34
Muscles; Heart
Applicants:
NIHON KOHDEN CORPORATION [JP/JP]; 31-4, Nishiochiai 1-chome, Shinjuku-ku, Tokyo 1618560, JP
TOKYO WOMEN'S MEDICAL UNIVERSITY [JP/JP]; 8-1, Kawada-cho, Shinjuku-ku, Tokyo 1628666, JP
Inventors:
KAGAWA Yuki; JP
SHIMIZU Tatsuya; JP
HARAGUCHI Yuji; JP
KUBO Hirotsugu; JP
Agent:
SHIN-EI PATENT FIRM, P.C.; Toranomon East Bldg. 8F, 7-13, Nishi-Shimbashi 1-chome, Minato-ku, Tokyo 1050003, JP
Priority Data:
2017-12787329.06.2017JP
Title (EN) METHOD FOR PRODUCING LAYERED CELL SHEET AND LAYERED CELL SHEET PRODUCED BY THE SAME
(FR) PROCÉDÉ DE PRODUCTION D'UNE FEUILLE DE CELLULES EN COUCHES ET FEUILLE DE CELLULES EN COUCHES PRODUITE PAR CELUI-CI
Abstract:
(EN) The present invention provides a method for swiftly producing a layered cell sheet that is non-invasively obtained and is utilizable for transplantation, etc., the method including (1) a step of applying a centrifugal force to a first cell sheet on a temperature-responsive culture surface for a predetermined time in a temperature range from a lower critical solution temperature of the temperature-responsive culture surface to 45℃, (2) a step of further placing a second cell sheet on the first cell sheet, and (3) a step of applying a centrifugal force to the first cell sheet and the second cell sheet on the temperature-responsive culture surface for a predetermined time in the temperature range from the lower critical solution temperature to 45℃; and also provides a layered cell sheet obtained by the method.
(FR) La présente invention concerne un procédé de production rapide d'une feuille de cellules en couches qui est obtenue de manière non-invasive et est utilisable pour une transplantation, etc., le procédé comprenant (1) une étape pour appliquer une force centrifuge à une première feuille de cellules sur une surface de culture sensible à la température pendant une durée prédéterminée dans une plage de température allant d'une température de solution critique inférieure de la surface de culture sensible à la température à 45 °C, (2) une étape pour placer en outre une seconde feuille de cellules sur la première feuille de cellules, et (3) une étape pour appliquer une force centrifuge à la première feuille de cellules et la seconde feuille de cellules sur la surface de culture sensible à la température pendant une durée prédéterminée dans la plage de température allant de la température de solution critique inférieure à 45 °C ; et concerne également une feuille de cellules en couches obtenue par le procédé.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)