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1. (WO2019003224) BIOCOMPATIBLE NANOFIBER ADHESIVE
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CLAIMS:

1. A nanofiber comprising a cross -linkable protein polymer and a transglutaminase.

2. The nanofiber according to claim 1, wherein the cross -linkable protein polymer comprises lysine and glutamine residues not in a cross-linked state.

3. The nanofiber according to claim 1, wherein at least 25%, at least 50%, at least 75%, or at least 90% of the lysine and glutamine residues comprised in the cross -linkable protein polymer are not in a cross -linked state.

4. The nanofiber according to claim 1, wherein the cross -linkable protein polymer comprises one or more selected from the group consisting of a fibrin, an elastin, an albumin, a gelatin and a collagen.

5. The nanofiber according to claim 1, wherein the cross -linkable protein polymer comprises a gelatin.

6. The nanofiber according to claim 1, wherein the weight ratio of TGase (mg) :gelatin (g) is between 1 mg : 1 g and 20 mg : 1 g.

7. The nanofiber according to claim 1, wherein he transglutaminase is a microbial transglutaminase.

8. A nanofiber mat comprising a plurality of nanofibers according to claim 1.

9. The nanofiber mat according to claim 7 that is operable to provide adhesion of at least 1 N as measured by a T-peel test between two wetted surfaces adhered to with the nanofiber mat, wherein the two wetted surfaces respectively comprises wetted collagen films having a width of 20 mm and length of 80mm, and the speed of peeling is 40 millimeters per minute.

10. The nanofiber mat according to 9, wherein the adhesion as measured by the T-peel test is at least 2 N.

11. A method for producing a nanofiber, the method comprising:

combining a first solution comprising a cross -linkable protein polymer and a second solution comprising a TGase to produce a liquid electro spinning mixture (LEM) characterized by an environment that is unsuitable for the TGase to be enzymatically active on the cross-linkable protein polymer; and

electro spinning the LEM to form a nanofiber comprising the TGase and the cross- linkable protein polymer.

12. The method according to claim 11, wherein the environment unsuitable for the TGase to be enzymatically active on the cross -linkable protein polymer is one or more of:

a pH of 5 or less;

a combined liquid composition of the LEM being less than 50% water and/or more than 50% organic solvent; and

the LEM being an emulsion comprising a first phase and a second phase, wherein the first solution is comprised in the first phase and second solution is compised in the second phase.

13. The method according to claim 11, wherein the first solution comprises the cross -linkable protein polymer dissolved in an acidic solvent.

14. The method according to claim 13, wherein the acidic solvent comprises acetic acid or formic acid.

15. The method according to claim 14, wherein the acidic solvent consists of glacial acetic acid.

16. The method according to claim 11, wherein the first solution comprises the cross -linkable protein polymer dissolved in an organic solvent.

17. The method according to claim 16, wherein the organic solvent is selected from the group consisting of: 2,2,2-Trifluoroethanol (TFE) and l,l,l,3,3,3-hexafluoro-2-propanol (HFIP).

18. The method according to claim 11, wherein the LEM comprises a surfactant.

19. The method according to claim 18, wherein the surfactant comprises one or a combination of two or more of: a cationic surfactant, an ionic surfactant, and a non-ionic surfactant.

20. The method according to claim 11, wherein the cross-linkable protein polymer comprises one or more selected from the group consisting of a fibrin, an elastin, an albumin, a gelatin and a collagen.

21. The method according to claim 11, wherein the cross-linkable protein polymer comprises a gelatin.

22. A nanofiber mat comprising:

a first nanofiber comprising a synthetic polymer and the transglutaminase; and

a second nanofiber comprising the cross-linkable protein polymer.

23. The nanofiber mat according to claim 22, wherein the synthetic polymer comprises a biocompatible polyester.

24. The nanofiber mat according to claim 22, wherein the synthetic polymer comprises a Polycaprolactone (PCL) or a poly (lactide-co-e-caprolactone) (PLCL).

25. The nanofiber mat according to claim 22, wherein the synthetic polymer comprises one or more of: a PC (polycarbonate); a PCU (polycarbonate uethane); a PDO,PDS (Polydioxanone); a PEO (poly ethylene oxide); a PES (polyesthersulfone); a PEVA (poluethylene-co- vinyl acetate); a PGA (polyglutamic acid); a PGCA (poly(glycolic acid); a PGS (poly(glycerol sebacate); a PHBV (Poly(3-hydroxybutyrate-co-3- hydroxy valerate); a PHB (Polyhydroxybutyrate); a PLA (Poly(L-lactic)); a PLA-PEG (D,L-Lactic -polyethylene glycol); a PLGA (poly(lactic-co-glycolic acid); a PLLA (poly- L-lactic acid); a PMMA (poly(methyl methacrylate); a PU (poly urethane); a PVA (poly vinyl alchohol); a PVAc (poly vinyl acetate); and a PVP (poly vinyl pryrrolidone).

26. The nanofiber mat according to claim 22, wherein the cross -linkable protein polymer comprises lysine and glutamine residues not in a cross-linked state.

27. The nanofiber mat according to claim 22, wherein at least 25%, at least 50%, at least 75%, or at least 90% of the lysine and glutamine residues comprised in the cross -linkable protein polymer are not in a cross-linked state.

28. The nanofiber mat according to claim 22, wherein the cross -linkable protein polymer comprises one or more selected from the group consisting of a fibrin, an elastin, an albumin, a gelatin and a collagen.

29. The nanofiber mat according to claim 22, wherein the cross -linkable protein polymer comprises a gelatin.

30. A method for producing a nanofiber mat, the method comprising:

electro spinning a first LEM comprising a cross -linkable protein polymer to form a first nanofiber;

electro spinning a second LEM comprising a TGase and a synthetic polymer to form a second a nanofiber; and

directing the first and second nanofibers to a common target surface, such that the first and second nanofibers commingle to form the nanofiber mat.

31. The method according to claim 30 wherein the cross -linkable protein polymer comprises one or more selected from the group consisting of a fibrin, an elastin, an albumin, a gelatin and a collagen.

32. The method according to claim 30, wherein the cross -linkable protein polymer comprises a gelatin.

33. The method according to claim 30, wherein the synthetic polymer comprises a biocompatible polyester.

34. The method according to claim 30, wherein the synthetic polymer comprises a Polycaprolactone (PCL) or a poly (lactide-co-e-caprolactone) (PLCL).

35. The method according to claim 30, wherein the synthetic polymer comprises one or more of: a PC (polycarbonate); a PCU (polycarbonate uethane); a PDO,PDS (Polydioxanone); a PEO (poly ethylene oxide); a PES (polyesthersulfone); a PEVA (poluethylene-co -vinyl acetate); a PGA (polyglutamic acid); a PGCA (poly(glycolic acid); a PGS (poly(glycerol sebacate); a PHBV (Poly(3-hydroxybutyrate-co-3-hydroxyvalerate); a PHB (Polyhydroxybutyrate); a PLA (Poly(L-lactic)); a PLA-PEG (D,L-Lactic-polyethylene glycol); a PLGA (poly(lactic-co-glycolic acid); a PLLA (poly-L-lactic acid); a PMMA (poly (methyl methacrylate); a PU (poly urethane); a PVA (poly vinyl alchohol); a PVAc (poly vinyl acetate); and a PVP (poly vinyl pryrrolidone).