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1. (WO2019002658) SYNTHESIS OF BIODIESEL CATALYSED BY AN ENZYMATIC CRUDE IMMOBILISED ON MAGNETIC PARTICLES
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Pub. No.: WO/2019/002658 International Application No.: PCT/ES2018/070466
Publication Date: 03.01.2019 International Filing Date: 28.06.2018
IPC:
C12N 11/14 (2006.01) ,C12P 7/64 (2006.01) ,C12N 9/18 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
11
Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
14
Enzymes or microbial cells being immobilised on or in an inorganic carrier
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7
Preparation of oxygen-containing organic compounds
64
Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
16
acting on ester bonds (3.1)
18
Carboxylic ester hydrolases
Applicants:
CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICAS (CSIC) [ES/ES]; C/ Serrano, nº 117 28006 Madrid, ES
Inventors:
PRIETO ORZANCO, Alicia Mª; ES
MOLINA GUTIÉRREZ, María; ES
MARTÍNEZ FERRER, Ángel T.; ES
MARTÍNEZ HERNÁNDEZ, Mª Jesús; ES
Agent:
PONS ARIÑO, Ángel; ES
Priority Data:
P20173085528.06.2017ES
Title (EN) SYNTHESIS OF BIODIESEL CATALYSED BY AN ENZYMATIC CRUDE IMMOBILISED ON MAGNETIC PARTICLES
(FR) SYNTHÈSE DE BIODIESEL CATALYSÉE PAR UN BRUT ENZYMATIQUE IMMOBILISÉ SUR DES PARTICULES MAGNÉTIQUES
(ES) SÍNTESIS DE BIODIESEL CATALIZADA POR UN CRUDO ENZIMÁTICO INMOVILIZADO SOBRE PARTÍCULAS MAGNÉTICAS
Abstract:
(EN) The present invention relates to a method for the enzymatic synthesis of long-chain fatty acid alkyl esters in the presence of an alcohol and of an enzymatic preparation comprising a sterol esterase/lipase immobilised covalently on surface-functionalised magnetic particles. The immobilisation of the versatile lipase using the method described can increase the yield of the alkyl ester synthesis process, as well as allowing the catalyst to be recovered for future reactions in a simple and efficient manner, without the activity and speed of the sterol esterase/lipase being reduced significantly during at least 10 consecutive reaction cycles.
(FR) La présente invention se rapporte à un procédé de synthèse enzymatique d'esters d'alkyle d'acides gras à chaîne longue en présence d'un alcool et d'une préparation enzymatique qui comprend un stérol estérase/lipase immobilisé de manière covalente sur des particules magnétiques fonctionnalisées sur la surface. L'immobilisation de la lipase transformable au moyen du procédé de l'invention peut augmenter le rendement du processus de synthèse d'esters d'alkyle, et peut également permettre une récupération, simple et efficace, du catalyseur pour des réactions ultérieures, sans que l'activité et la vitesse de la stérol estérase/lipase se trouvent réduites notablement sur au moins 10 cycles de réaction consécutifs.
(ES) La presente invención se refiere a un procedimiento para la síntesis enzimática de ésteres de alquilo de ácidos grasos de cadena larga en presencia de un alcohol y de una preparación enzimática que comprende una esterol esterasa/lipasa inmovilizada covalentemente sobre partículas magnéticas funcionalizadas en su superficie. La inmovilización de la lipasa versátil mediante el procedimiento descrito es capaz de incrementar el rendimiento del proceso de síntesis de ésteres de alquilo, así como de permitir una recuperación del catalizador para futuras reacciones, sencilla y eficiente, sin que la actividad y velocidad de la esterol esterasa/lipasa se vea disminuida notablemente a lo largo de al menos 10 ciclos de reacción consecutivos.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Spanish (ES)
Filing Language: Spanish (ES)