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1. (WO2019001187) MULTI-LIQUID PHASE GENE CHIP DETECTION PRIMER, KIT AND METHOD FOR RAPIDLY DISTINGUISHING FIVE PATHOGENS IN MOUSE RESPIRATORY TRACTS
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Pub. No.: WO/2019/001187 International Application No.: PCT/CN2018/088302
Publication Date: 03.01.2019 International Filing Date: 25.05.2018
IPC:
C12Q 1/70 (2006.01) ,C12Q 1/68 (2018.01) ,C12Q 1/04 (2006.01) ,C12N 15/11 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
70
involving virus or bacteriophage
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
04
Determining presence or kind of micro-organism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
Applicants:
广东省实验动物监测所 GUANGDONG LABORATORY ANIMALS MONITORING INSTITUTE [CN/CN]; 中国广东省广州市 黄埔区风信路11号王海曼 WANG, Haiman Fengxin Road No. 11, Huangpu District Guangzhou, Guangdong 510663, CN
Inventors:
王静 WANG, Jing; CN
郭鹏举 GUO, Pengju; CN
黄韧 HUANG, Ren; CN
张钰 ZHANG, Yu; CN
Agent:
广州市科丰知识产权代理事务所(普通合伙) GUANGZHOU KEFENG INTELLECTUAL PROPERTY AGENCY (GENERAL PARTNERSHIP); 中国广东省广州市 黄埔区黄埔东路5号2618房王海曼 WANG, Haiman Room 2618, No. 5 Huangpu East Road Huangpu District, Guangzhou Guangdong 510032, CN
Priority Data:
201710505600.728.06.2017CN
Title (EN) MULTI-LIQUID PHASE GENE CHIP DETECTION PRIMER, KIT AND METHOD FOR RAPIDLY DISTINGUISHING FIVE PATHOGENS IN MOUSE RESPIRATORY TRACTS
(FR) AMORCE DE DÉTECTION DE PUCE À ADN DE PHASE MULTI LIQUIDE, KIT ET MÉTHODE POUR DISTINGUER RAPIDEMENT CINQ PATHOGÈNES DANS DES VOIES RESPIRATOIRES DE SOURIS
(ZH) 一种快速区分五种小鼠呼吸道病原的多重液相基因芯片检测引物、试剂盒及方法
Abstract:
(EN) Disclosed are a multi-liquid phase gene chip detection primer, a kit and a method for rapidly distinguishing five pathogens in mouse respiratory tracts. The present invention obtains a target amplified fragment by PCR, and then hybridizes the amplified product, a fluorescence-encoded microsphere and streptavidin-phycoerythrin, and reads the MFI value via a detector to distinguish different types of viruses.
(FR) L'invention concerne une amorce de détection de puce à ADN de phase multi-liquide, un kit et une méthode pour distinguer rapidement cinq pathogènes dans des voies respiratoires de souris. La présente invention obtient un fragment cible amplifié par PCR, puis hybride le produit amplifié, une microsphère codée par fluorescence et de la streptavidine-phycoérythrine, et lit la valeur MFI par l'intermédiaire d'un détecteur pour distinguer différents types de virus.
(ZH) 本发明公开了一种快速区分5种小鼠呼吸道病原的多重液相基因芯片检测引物、试剂盒及方法。本发明通过PCR获得目标扩增片段,然后将扩增产物、荧光编码微球和链霉亲和素‑藻红蛋白进行杂交,通过检测仪读取MFI值,从而分辨不同类型的病毒。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)