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1. (WO2019000806) METHOD FOR CREATING MALE STERILITY LINE OF SOLANUM LYCOPERSICUM BY MEANS OF GENOME EDITING, AND APPLICATION THEREOF
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Pub. No.: WO/2019/000806 International Application No.: PCT/CN2017/111859
Publication Date: 03.01.2019 International Filing Date: 20.11.2017
IPC:
C12N 15/82 (2006.01) ,C12N 15/29 (2006.01) ,A01H 5/00 (2018.01) ,C12Q 1/68 (2018.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
82
for plant cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
29
Genes encoding plant proteins, e.g. thaumatin
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
H
NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5
Flowering plants, i.e. angiosperms
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
北京市农林科学院 BEIJING ACADEMY OF AGRICULTURE AND FORESTRY SCIENCES [CN/CN]; 中国北京市 海淀区曙光花园中路11号 No.11 Middle Road Shuguang Garden, Haidian District Beijing 100097, CN
中国科学院遗传与发育生物学研究所 INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCES [CN/CN]; 中国北京市 朝阳区北辰西路1号院 No.1 West Beichen Road, Chaoyang district Beijing 100101, CN
Inventors:
李常保 LI, Changbao; CN
杜敏敏 DU, Minmin; CN
李传友 LI, Chuanyou; CN
邓磊 DENG, Lei; CN
周明 ZHOU, Ming; CN
温常龙 WEN, Changlong; CN
Agent:
北京纪凯知识产权代理有限公司 JEEKAI & PARTNERS; 中国北京市 丰台区广安路9号5号楼15A层 Floor 15A, Building No.5 9 Guang'an Road, Fengtai District Beijing 100055, CN
Priority Data:
201710514085.929.06.2017CN
Title (EN) METHOD FOR CREATING MALE STERILITY LINE OF SOLANUM LYCOPERSICUM BY MEANS OF GENOME EDITING, AND APPLICATION THEREOF
(FR) PROCÉDÉ DE CRÉATION D'UNE LIGNÉE MÂLE STÉRILE DE SOLANUM LYCOPERSICUM PAR ÉDITION DU GÉNOME, ET APPLICATION ASSOCIÉE
(ZH) 一种通过基因组编辑创制番茄雄性不育系的方法及其应用
Abstract:
(EN) Provided is a method for rapidly creating a male sterility line of Solanum lycopersicum using the CRISPR/Cas9 genome editing technology, comprising editing gene Solyc03g053130 of Solanum lycopersicum using the CRISPR/Cas9 technology, so as to obtain, by means of selfing, a transgenic homozygous male sterile mutant which does not contain CAS9. Also provided is a method for assisting in identifying a male sterile plant, relating to detecting the genotype of SNP1606 in gene Solyc03g053130 in a Solanum lycopersicum genome. If the genotype of SNP1606 in a genome of Solanum lycopersicum to be detected is T/T homozygous, the Solanum lycopersicum to be detected is a male sterile plant or a candidate male sterile plant.
(FR) L'invention concerne un procédé pour créer rapidement une lignée mâle stérile de Solanum lycopersicum à l'aide de la technologie d'édition du génome CRISPR/cas9, comprenant l'édition du gène Solyc03g053130 de Solanum lycopersicum à l'aide de la technologie CRISPR/cas9, de façon à obtenir, par autofécondation, un mutant mâle stérile homozygote transgénique qui ne contient pas CAS9. L'invention concerne également un procédé d'aide à l'identification d'une plante mâle stérile, se rapportant à la détermination du génotype du SNP1606 dans le gène Solyc03g053130 du génome de Solanum lycopersicum. Si le génotype du SNP1606 du génome de Solanum lycopersicum à déterminer est homozygote T/T, le Solanum lycopersicum à déterminer est une plante mâle stérile ou une plante mâle stérile candidate.
(ZH) 提供了一种利用CRISPR/Cas9基因组编辑技术快速创制番茄雄性不育系的方法,利用CRISPR/Cas9技术对番茄Solyc03g053130基因进行编辑,进而通过自交获得纯合且不含CAS9转基因的雄性不育突变体。还提供了一种辅助鉴定雄性不育株的方法,是检测番茄基因组中Solyc03g053130基因中SNP1606的基因型,若待测番茄基因组SNP1606的基因型为T/T纯合,则待测番茄为或候选为雄性不育株。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)