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1. (WO2019000158) TUNNEL IDENTIFICATION TECHNOLOGY-BASED NANO DETECTION DEVICE AND METHOD
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Pub. No.: WO/2019/000158 International Application No.: PCT/CN2017/089972
Publication Date: 03.01.2019 International Filing Date: 26.06.2017
IPC:
C12Q 1/68 (2018.01) ,G01N 27/26 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27
Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
26
by investigating electrochemical variables; by using electrolysis or electrophoresis
Applicants:
武汉科技大学 WUHAN UNIVERSITY OF SCIENCE AND TECHNOLOGY [CN/CN]; 中国湖北省武汉市 青山区和平大道947号武汉科技大学材料与治金学院 School of Materials and Metallurgy,Wuhan University of Science and Technology 947 Heping Avenue,Qingshan District Wuhan City, Hubei 430081, CN
Inventors:
常帅 CHANG, Shuai; CN
何劲 HE, Jin; CN
黄明柱 HUANG, Mingzhu; CN
顾华志 GU, Huazhi; CN
Agent:
北京轻创知识产权代理有限公司 KEYCOM PARTNERS, P.C.; 中国北京市 海淀区莱圳家园18号楼3层3011室 Room 3011, Xinhua Innovation Building, No.18 Laizhenjiayuan, Haidian District Beijing 100192, CN
Priority Data:
Title (EN) TUNNEL IDENTIFICATION TECHNOLOGY-BASED NANO DETECTION DEVICE AND METHOD
(FR) DISPOSITIF ET PROCÉDÉ DE NANO-DÉTECTION BASÉS SUR LA TECHNOLOGIE D'IDENTIFICATION DE TUNNEL
(ZH) 一种基于隧道识别技术的纳米检测装置及方法
Abstract:
(EN) A tunnel identification technology-based nano detection device and method. The detection device comprises a NanoPen (1), a planar electrode (2), an active power supply (4) and a current tester (3). The detection method comprises the following steps: placing the NanoPen (1) in a test solution (5) having a specific DNA or RNA sequence, and placing the planar electrode (2) on the surface of the test solution (5); introducing the DNA or RNA sequence into a delivery pipeline (12); detecting and recording a current change displayed by the current tester (3); and reading a base signal of the DNA or RNA sequence according to the detected current change. The described technical solution is a technique for directly reading a DNA sequence, which has the characteristics of fast reading speed and high accuracy.
(FR) L'invention concerne un dispositif et un procédé de nano-détection basés sur une technologie d'identification de tunnel. Le dispositif de détection comprend un nano-stylo (1), une électrode plane (2), une alimentation électrique active (4) et un testeur de courant (3). Le procédé de détection comprend les étapes suivantes : placer le nano-stylo (1) dans une solution de test (5) ayant une séquence d'ADN ou d'ARN spécifique, et placer l'électrode plane (2) sur la surface de la solution de test (5); introduire la séquence d'ADN ou d'ARN dans un conduit de distribution (12); détecter et enregistrer un changement de courant affiché par le testeur de courant (3); et lire un signal de base de la séquence d'ADN ou d'ARN selon le changement de courant détecté. La solution technique décrite est une technique de lecture directe d'une séquence d'ADN, qui présente les caractéristiques d'une vitesse de lecture rapide et d'une haute précision.
(ZH) 一种基于隧道识别技术的纳米检测装置及方法。所述检测装置包括纳米笔(1),平面电极(2)、有源电源(4)及电流测试仪(3)。所述检测方法包括如下步骤:将所述纳米笔(1)放置于具有特定DNA或RNA序列的测试溶液(5)中,将所述平面电极(2)放置于所述测试溶液(5)表面;将所述DNA或RNA序列导入所述输送管道(12);检测并记录所述电流测试仪(3)显示的电流变化;根据检测的所述电流变化读取所述DNA或RNA序列的碱基信号。该技术方案为DNA序列直接读取技术,具有读取速度快,准确率高的特点。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)