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1. (WO2019000147) TUD RNA EFFECTIVELY INHIBITING EXPRESSION OF HUMAN MIR-148A, MIR-152 AND MIR-185 AND USE THEREOF
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Pub. No.: WO/2019/000147 International Application No.: PCT/CN2017/089927
Publication Date: 03.01.2019 International Filing Date: 26.06.2017
IPC:
C12N 15/113 (2010.01) ,C12N 15/66 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
113
Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
66
General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
Applicants:
深圳市博奥康生物科技有限公司 SHENZHEN BIOCAN TECHNOLOGIES CO., LTD [CN/CN]; 中国广东省深圳市 南山区珠光路134号珠光创新科技园1栋509毛吉炎 MAO, Jiyan Room 509, Building 1, Zhuguang Innovation Park, No. 134 Zhuguang Road, Nanshan District Shenzhen, Guangdong 518055, CN
Inventors:
毛吉炎 MAO, Jiyan; CN
Priority Data:
Title (EN) TUD RNA EFFECTIVELY INHIBITING EXPRESSION OF HUMAN MIR-148A, MIR-152 AND MIR-185 AND USE THEREOF
(FR) ARN TUD INHIBANT EFFICACEMENT L'EXPRESSION DES MIR-148A, MIR-152 ET MIR-185 HUMAINS ET SON UTILISATION
(ZH) 一种有效抑制人MIR-148A、MIR-152和MIR-185表达的TUD RNA及其应用
Abstract:
(EN) Provided are a TuD RNA and the use thereof. The TuD RNA can effectively inhibit the expression of human miR-148a, miR-152 and miR-185. The DNA sequence encoding the TuD RNA is as shown below: 5'-ggcgctaggatcatcaactccccgaccgaaatctaggagaccagcaaagtcacgtactatctgtcttgaacccaaagtcacgtgatgatcttcttgaaacacaagtattctggtcacagaatacaactccccgaccgaaatctaggagaccagcaaagtcacgtactatctgtcttgaacccaaagtcacgtgatgatcttcttgaaacacaagatgatcctagcgccaccttttt-3'. The use of the TuD RNA comprises constructing the TuD RNA on a lentiviral vector to obtain a recombinant vector; and applying the recombinant vector to the human cells to inhibit the expression of human miR-148a, miR-152 and miR-185.
(FR) L'invention concerne un ARN TuD et son utilisation. L'ARN TuD peut inhiber efficacement l'expression des miR-148a, miR-152 et miR-185 humains. La séquence d'ADN codant pour l'ARN TuD est telle que représentée ci-dessous : 5'-ggcgctaggatcatcaactccccgaccgaaatctaggagaccagcaaagtcacgtactatctgtcttgaacccaaagtcacgtgatgatcttcttgaaacacaagtattctggtcacagaatacaactccccgaccgaaatctaggagaccagcaaagtcacgtactatctgtcttgaacccaaagtcacgtgatgatcttcttgaaacacaagatgatcctagcgccaccttttt-3'. L'utilisation de l'ARN TuD consiste à construire l'ARN TuD sur un vecteur lentiviral pour obtenir un vecteur recombiné; et à appliquer le vecteur recombiné aux cellules humaines pour inhiber l'expression des miR-148a, miR-152 et miR-185 humains.
(ZH) 提供一种Tud RNA及其应用,该Tud RNA可以有效抑制人miR-148a、miR-152和miR-185表达。编码所述Tud RNA的DNA序列,如下所示:5'-ggcgctaggatcatcaactccccgaccgaaatctaggagaccagcaaagtcacgtactatctgtcttgaacccaaagtcacgtgatgatcttcttgaaacacaagtattctggtcacagaatacaactccccgaccgaaatctaggagaccagcaaagtcacgtactatctgtcttgaacccaaagtcacgtgatgatcttcttgaaacacaagatgatcctagcgccaccttttt-3'。该Tud RNA的应用,包括将该Tud RNA构建于慢病毒载体上,得到重组载体;将该重组载体作用于人源细胞,抑制人miR-148a、miR-152和miR-185的表达。
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African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)