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1. (WO2019000144) CHO CELL EXPRESSING AITR GENE AND USE THEREOF
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Pub. No.: WO/2019/000144 International Application No.: PCT/CN2017/089924
Publication Date: 03.01.2019 International Filing Date: 26.06.2017
IPC:
C12N 15/57 (2006.01) ,C12N 15/64 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
52
Genes encoding for enzymes or proenzymes
55
Hydrolases (3)
57
acting on peptide bonds (3.4)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
64
General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
Applicants:
深圳市博奥康生物科技有限公司 SHENZHEN BIOCAN TECHNOLOGIES CO.,LTD. [CN/CN]; 中国广东省深圳市 南山区西丽街道珠光路134号珠光创新科技园1栋509室毛吉炎 Jiyan MAO Room 509, Building 1, Zhuguang Innovation Park, No.134 Zhuguang Road, Xilii Street, Nanshan District Shenzhen, Guangdong 518055, CN
Inventors:
毛吉炎 MAO, Jiyan; CN
Priority Data:
Title (EN) CHO CELL EXPRESSING AITR GENE AND USE THEREOF
(FR) CELLULE CHO EXPRIMANT LE GÈNE AITR ET UTILISATION ASSOCIÉE
(ZH) 一种表达AITR基因的CHO细胞及其应用
Abstract:
(EN) Disclosed is a CHO cell expressing the AITR gene. The full-length AITR gene eukaryotic expression vector pCAG(m)-IRESblast-AITR is constructed using the eukaryotic expression vector pCAG(m)-IRESblast and is further used to transfect the CHO cells, and the blasticidin is used to screen same to obtain the engineered cell line CHO/AITR highly expressing the full-length AITR gene, and it is confirmed that the cell line can greatly increase the expression of the full-length AITR.
(FR) L'invention concerne une cellule CHO exprimant le gène AITR. Le vecteur d'expression eucaryote du gène AITR pleine longueur pCAG(m)-IRESblast-AITR est construit à l'aide du vecteur d'expression eucaryote pCAG(m)-IRESblast et est en outre utilisé pour transfecter les cellules CHO, et la blasticidine est utilisée pour cribler celles-ci pour obtenir la lignée cellulaire modifiée CHO/AITR exprimant fortement le gène AITR pleine longueur, et il a été confirmé que la lignée cellulaire peut considérablement augmenter l'expression de l'AITR pleine longueur.
(ZH) 一种表达AITR基因的CHO细胞,利用真核表达载体pCAG(m)-IRESblast构建全长AITR基因真核表达载体pCAG(m)-IRESblast-AITR,进而以其转染CHO细胞,利用杀稻瘟菌素筛选得到高表达全长AITR的基因的工程细胞系CHO/AITR,并证实该细胞系可大幅提高全长AITR的表达。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)