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1. (WO2018228246) METHOD FOR ENZYMATIC PREPARATION OF GLUTATHIONE
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Pub. No.: WO/2018/228246 International Application No.: PCT/CN2018/090045
Publication Date: 20.12.2018 International Filing Date: 06.06.2018
IPC:
C12P 21/02 (2006.01) ,A61K 38/06 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21
Preparation of peptides or proteins
02
having a known sequence of two or more amino acids, e.g. glutathione
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38
Medicinal preparations containing peptides
04
Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
06
Tripeptides
Applicants:
安徽古特生物科技有限公司 ANHUI GSH BIO-TECH CO., LTD [CN/CN]; 中国安徽省安庆市 大观区环城西路99号凤凰公寓4栋1单元1003室 Fenghuang Building 4-1-1003 Huanchengxi Road 99, Daguan District Anqing, Anhui 246000, CN
Inventors:
刘珊珊 LIU, Shanshan; CN
刘辉 LIU, Hui; CN
周稳文 ZHOU, Wenwen; CN
秦永发 QIN, Yongfa; CN
Agent:
北京金岳知识产权代理事务所(特殊普通合伙) CHINA KINTEC IP LAWS LLP; 中国北京市 房山区书院南街10号院11号楼8层818王文生 WANG, Wensheng 818 8/F, Building 11, Yard No.10 of College South Street, Fangshan District Beijing 102488, CN
Priority Data:
201710452240.915.06.2017CN
Title (EN) METHOD FOR ENZYMATIC PREPARATION OF GLUTATHIONE
(FR) MÉTHODE DE PRÉPARATION ENZYMATIQUE DE GLUTATHION
(ZH) 一种酶法制备谷胱甘肽的方法
Abstract:
(EN) Disclosed is a method for enzymatic preparation of glutathione, comprising the following steps: (1) utilizing a GshF enzyme, an ATP regenerating enzyme, and an AK enzyme in a reactor to produce glutathione; (2) separating the solidified GshF enzyme, ATP regenerating enzyme, and AK enzyme in the reactor, or using a filtration apparatus to separate free GshF enzyme; (3) separating the filtrate produced in step (2) to produce a crude product GSH and traces of ATP, ADP, and AMP; and (4) concentrating, crystallizing, and drying the GSH produced in step (3) to produce a product GSH.
(FR) L'invention concerne une méthode de préparation enzymatique de glutathion, comprenant les étapes suivantes : (1) l'utilisation d'une enzyme GshF, d'une enzyme de régénération d'ATP et d'une enzyme AK dans un réacteur pour produire du glutathion ; (2) la séparation de l'enzyme GshF, de l'enzyme de régénération d'ATP et de l'enzyme AK solidifiées dans le réacteur, ou l'utilisation d'un appareil de filtration pour séparer l'enzyme GshF libre ; (3) la séparation du filtrat produit à l'étape (2) pour produire un produit brut de GSH et des traces d'ATP, d'ADP et d'AMP ; et (4) la concentration, la cristallisation et le séchage du GSH produit à l'étape (3) pour produire un produit de GSH.
(ZH) 公开了一种酶法制备谷胱甘肽的方法,包括以下步骤:(1)利用GshF酶、ATP再生酶和AK酶在反应器中生成谷胱甘肽;(2)在反应器中分离固定化的GshF酶、ATP再生酶和AK酶,或使用过滤设备分离游离的GshF酶;(3)将步骤(2)得到的滤出液进行分离,得粗产物GSH以及少量的ATP、ADP和AMP;(4)将步骤(3)得到的GSH通过浓缩、结晶、干燥制备成品GSH。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)