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1. (WO2018226171) MUTANT STRAIN ASPERGILLUS ACULEATUS FOR PRODUCING CELLULASE AND XYLANASE AND PREPARATION METHOD THEREOF
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Claims

1. A mutant strain Aspergillus aculeatus El 4-292, accession number NITE ABP-02391, for producing cellulase and xylanase, wherein said strain produced from chemical mutagens genetic modification.

2. A genetic modification process to produce the mutant strain Aspergillus aculeatus

El 4-292 according to claim 1, wherein said process comprises the following step:

a) contacting the Aspergillus aculeatus BCC199 (wild type) to methylethanesulfonate (EMS) at ratio of methylethanesulfonate to fungi in a range of 20 - 30 mM to 107 spores from 1 to 3 hours;

b) cultivating the mutant strain Aspergillus aculeatus produced from a); and c) isolating the mutant strain Aspergillus aculeatus cultivated from step b).

3. The genetic modification process according to claim 2, wherein step a) the ratio of methylethanesulfonate to fungi is in a range of 25 - 26 mM to 107 spores.

4. The genetic modification process according to claim 2 or 3, wherein step a) is operated for 3 hours.

5. The genetic modification process according to any one of claims 2 - 4, wherein step a) further comprises the adding step of detoxifying agent selected from sodium hydroxide or sodium thiosulfate.

6. The genetic modification process according to claim 5, wherein the detoxifying agent is sodium thiosulfate (Na2S203).

7. The genetic modification process according to any one of claims 2 - 6, wherein said process is operated twice.

8. A production process of cellulase and xylanase by solid state fermentation process comprising the step of cultivating the mutant strain Aspergillus aculeatus El 4-292 according to claim 1, wherein the ratio of the mutant strain Aspergillus aculeatus El 4-292 to cultivating medium is in a range of 109 - 1011 spores/kg under the temperature from 25 - 32 °C for 72 - 120 hours.

9. The production process of cellulase and xylanase by solid state fermentation process according to claim 8, wherein the ratio of the mutant strain Aspergillus aculeatus El 4-292 to cultivating medium is in the range of 1 x 1010 - 5 x 1010 spores/kg.

10. The production process of cellulase and xylanase by solid state fermentation process according to claim 8, wherein said process is operated at the temperature of 30 °C.

11. The production process of cellulase and xylanase by solid state fermentation process according to claim 8, wherein said process is operated for 96 hours.

12. The production process of cellulase and xylanase by submerged fermentation process comprises the following step:

a) preparing seed inoculum by cultivating the mutant strain Aspergillus aculeatus E14-292 in medium, wherein the ratio of the mutant strain Aspergillus aculeatus E14-292 to cultivating medium is in the range of 107 - 109 spores/L under the temperature from 25 - 32 °C for 36 - 60 hours; and

b) transferring seed inoculum obtained from a) into cultivating medium to reach the concentration of 10 - 15 % (w/v) and fermenting under the temperature from 25 - 32 °C for 96 - 144 hours.

13. The production process of cellulase and xylanase by submerged fermentation process according to claim 12, wherein the ratio of the mutant strain Aspergillus aculeatus El 4-292 to cultivating medium in step a) is in the range of 1 x 108 - 5 x 108 spores/L.

14. The production process of cellulase and xylanase by submerged fermentation process according to claim 12, wherein step a) is operated at the temperature of 30 °C.

15. The production process of cellulase and xylanase by submerged fermentation process according to claim 12, wherein step a) is operated for 48 hours.

16. The production process of cellulase and xylanase by submerged fermentation process according to claim 12, wherein the concentration of the seed inoculum in step b) is in the range of 12 - 13 % (w/v).

17. The production process of cellulase and xylanase by submerged fermentation process according to claim 12, wherein step b) is operated in the temperature of 30 °C.

18. The production process of cellulase and xylanase by submerged fermentation process according to claim 12, wherein step b) is operated for 120 hours.

19. The production process of cellulase and xylanase by submerged fermentation process according to any one of claims 8 - 18, wherein the carbon source is selected from cellulose powder or lignocellulosic materials such as rice straw, bagasse, corncob, corn stem.

20. The production process of cellulase and xylanase by submerged fermentation process according to claim 19, wherein the carbon source is corncob.

21. The use of enzymes produced from process according to any one of claims 8 - 20 for the saccharification of biomass.

22. The use of enzymes according to claim 21 for the saccharification of bagasse.