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1. (WO2018223142) CELL COLONY PICKING SYSTEM

Pub. No.:    WO/2018/223142    International Application No.:    PCT/US2018/035901
Publication Date: Fri Dec 07 00:59:59 CET 2018 International Filing Date: Tue Jun 05 01:59:59 CEST 2018
IPC: C12M 1/34
C12M 1/26
G01N 21/00
G01N 33/52
G06T 15/00
G06T 15/08
Applicants: MOLECULAR DEVICES, LLC
FLOTO, Timothy, A.
GLASER, Alison, A.
VON LANDAU, Lee
PHILLIPS, John
COREY, Kyle
Inventors: FLOTO, Timothy, A.
GLASER, Alison, A.
VON LANDAU, Lee
PHILLIPS, John
COREY, Kyle
Title: CELL COLONY PICKING SYSTEM
Abstract:
Systems, including methods and apparatus, for growing viable monoclonal colonies, identifying them as monoclonal after their viability has been demonstrated, and picking and placing them into a target environment. The methods may include plating cells at low density in a well, capturing at a first time shortly after plating a series of vertically spaced-apart images of the well showing locations of the cells, capturing at a second, later time images of the same well showing locations of candidate cell colonies, determining a likelihood that the candidate colonies are monoclonal based on information in the first set of images, and, based on the likelihood, picking candidate colonies using a picking head for transfer to a target environment.