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1. (WO2018223076) METHODS FOR ABSOLUTE QUANTIFICATION OF LOW-ABUNDANCE POLYPEPTIDES USING MASS SPECTROMETRY
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Pub. No.: WO/2018/223076 International Application No.: PCT/US2018/035716
Publication Date: 06.12.2018 International Filing Date: 01.06.2018
IPC:
G01N 33/68 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68
involving proteins, peptides or amino acids
Applicants:
GENZYME CORPORATION [US/US]; 500 Kendall Street Cambridge, Massachusetts 02142, US
Inventors:
STAPELS, Martha; US
BUSCH, Michelle; US
Agent:
JARECKI, Jessica; US
CHAPMAN, John D.; US
ZHOU, Jie; US
Priority Data:
62/514,58702.06.2017US
Title (EN) METHODS FOR ABSOLUTE QUANTIFICATION OF LOW-ABUNDANCE POLYPEPTIDES USING MASS SPECTROMETRY
(FR) PROCÉDÉS DE QUANTIFICATION ABSOLUE DE POLYPEPTIDES À FAIBLE ABONDANCE À L'AIDE DE LA SPECTROMÉTRIE DE MASSE
Abstract:
(EN) The present invention provides methods for improved label-free absolute quantification of relatively low abundant polypeptides by liquid chromatography/mass spectrometry analysis of peptide products obtained from simple or complex polypeptide mixtures. The methods for absolute quantification include MS signals from a set of qualified ions of peptide products of a relatively high abundant polypeptide to improve quantification of a relatively low abundant polypeptide.
(FR) La présente invention concerne des procédés permettant une quantification absolue sans marque améliorée de polypeptides relativement peu abondants par l'analyse par chromatographie liquide/spectrométrie de masse de produits peptidiques obtenus à partir de mélanges polypeptidiques simples ou complexes. Les procédés de quantification absolue comprennent des signaux MS provenant d'un ensemble d'ions qualifiés de produits peptidiques d'un polypeptide relativement très abondant afin d'améliorer la quantification d'un polypeptide relativement peu abondant.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)