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1. (WO2018222800) OLIGONUCLEOTIDE PROBE ARRAY WITH ELECTRONIC DETECTION SYSTEM

Pub. No.:    WO/2018/222800    International Application No.:    PCT/US2018/035282
Publication Date: Fri Dec 07 00:59:59 CET 2018 International Filing Date: Fri Jun 01 01:59:59 CEST 2018
IPC: C12Q 1/68
G06F 19/20
Applicants: CENTRILLION TECHNOLOGIES, INC.
Inventors: ZHOU, Wei
MCGALL, Glenn
MEI, Rui
CRNOGORAC, Filip
Title: OLIGONUCLEOTIDE PROBE ARRAY WITH ELECTRONIC DETECTION SYSTEM
Abstract:
The present disclosure provides methods, device, and system for a hybridization assay to detect nucleic acid targets. The assay may use a probe array format with addressable microwells. Each microwell may retain a plurality of copies of one probe which may be complementary to a portion of a specific nucleic acid target. The plurality of copies of probes may be produced from a pooled library of probe sequences by amplification techniques, including, rolling circle amplification and emulsion-PCR. Detection of hybridization may rely on methods using ion-sensitive field effect transistor (ISFET) and optical detectors to detect signals indicating the presence of the nucleic acid targets.