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1. (WO2018222667) GENOME EDITING METHODS FOR PRODUCING LOW-NICOTINE TOBACCO PRODUCTS

Pub. No.:    WO/2018/222667    International Application No.:    PCT/US2018/035058
Publication Date: Fri Dec 07 00:59:59 CET 2018 International Filing Date: Thu May 31 01:59:59 CEST 2018
IPC: C12N 15/82
C12N 15/10
C12N 15/113
C12N 9/10
C12N 9/22
Applicants: 22ND CENTURY LIMITED, LLC
Inventors: RUSHTON, Paul
Title: GENOME EDITING METHODS FOR PRODUCING LOW-NICOTINE TOBACCO PRODUCTS
Abstract:
The present technology provides targeted genome engineering (also known as genome editing) techniques to modify nicotine biosynthesis. In particular, the present technology relates to the use of genome editing methods to generate mutations resulting in an out-of-frame start codon upstream of the open reading frames (ORFs) of genes of interest, such as nicotine biosynthesis genes, to genetically engineer protein translation levels and modulate nicotine production in plants for producing plants and plant cells having reduced nicotine content.