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1. (WO2018220835) METHOD FOR SEPARATING RARE CELLS IN BLOOD SAMPLE AND METHOD FOR ANALYZING GENES OF SAID CELLS

Pub. No.:    WO/2018/220835    International Application No.:    PCT/JP2017/020661
Publication Date: Fri Dec 07 00:59:59 CET 2018 International Filing Date: Sat Jun 03 01:59:59 CEST 2017
IPC: C12N 5/09
C12Q 1/68
G01N 33/48
C12M 1/26
Applicants: HITACHI CHEMICAL COMPANY, LTD.
日立化成株式会社
Inventors: NAKAMURA Seita
中村 清太
ENDOH Katsuya
遠藤 勝也
Title: METHOD FOR SEPARATING RARE CELLS IN BLOOD SAMPLE AND METHOD FOR ANALYZING GENES OF SAID CELLS
Abstract:
This method for separating rare cells in a blood sample comprises: a step for filtering the blood sample by using a filter and capturing cells on the filter; and a step for bringing the cells captured on the filter into contact with a hemolytic agent. The hemolytic agent includes ammonium chloride or a cyclic amine. The rare cells separated according to this invention can be subjected to genetic analysis efficiently.