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1. (WO2018220644) METHOD OF PREPARATION AND USE OF POLYDIACETYLENE-BASED NANOPARTICLES FOR THE SENSING OF ANALYTES

Pub. No.:    WO/2018/220644    International Application No.:    PCT/IN2018/050340
Publication Date: Fri Dec 07 00:59:59 CET 2018 International Filing Date: Wed May 30 01:59:59 CEST 2018
IPC: C07C 233/00
G01N 33/577
G01N 33/531
Applicants: ADHIKARY, Rishi
Inventors: ADHIKARY, Rishi
SHAH, Diti
Title: METHOD OF PREPARATION AND USE OF POLYDIACETYLENE-BASED NANOPARTICLES FOR THE SENSING OF ANALYTES
Abstract:
The invention provides lipo-polymeric nanoparticles and their preparation. The process involves dissolving polydiacetylene in dimethyl sulfoxide and a phospholipid in chloroform followed by adding 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-Hydroxysuccinimide (NHS) in 1: 1 ratio, at least one polyclonal or monoclonal antibody, 1-20 picomoles of a cationic molecule and keeping said sample overnight at 2-8°C, followed by keeping under UV light at intervals of 10 seconds for a total of 30-60 seconds to allow the polydiacetylene to polymerize and form lipo-polymeric nanoparticles. The invention also provides a method of detection of an analyte comprising contacting a sample with lipo-polymeric nanoparticles, wherein presence of an analyte such as Alpha-fetoprotein (AFP); and beta-human chorionic gonadotropin (HCG) in sample analysed by a shift in color from blue phase to red phase with an absorption maximum at about 540 nm.