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1. (WO2018220582) METHODS OF SELECTING CELLS COMPRISING GENOME EDITING EVENTS

Pub. No.:    WO/2018/220582    International Application No.:    PCT/IB2018/053905
Publication Date: Fri Dec 07 00:59:59 CET 2018 International Filing Date: Fri Jun 01 01:59:59 CEST 2018
IPC: C12N 15/10
C12N 15/82
Applicants: TROPIC BIOSCIENCES UK LIMITED
Inventors: MAORI, Eyal
GALANTY, Yaron
PIGNOCCHI, Cristina
CHAPARRO GARCIA, Angela
MEIR, Ofir
Title: METHODS OF SELECTING CELLS COMPRISING GENOME EDITING EVENTS
Abstract:
Nucleic acid constructs for use in a method of selecting cells comprising a genome editing event, the method comprising (a) transforming cells of a plant of interest with the nucleic acid construct; (b) selecting transformed cells exhibiting fluorescence emitted by the fluorescent reporter using flow cytometry or imaging; and (c) culturing the transformed cells comprising the genome editing event by the DNA editing agent for a time sufficient to lose expression of the DNA editing agent so as to obtain cells which comprise a genome editing event generated by the DNA editing agent but lack DNA encoding the DNA editing agent.