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1. (WO2018220179) CRYOPRESERVATION

Pub. No.:    WO/2018/220179    International Application No.:    PCT/EP2018/064475
Publication Date: Fri Dec 07 00:59:59 CET 2018 International Filing Date: Sat Jun 02 01:59:59 CEST 2018
IPC: A01N 1/02
A61L 27/38
Applicants: UCL BUSINESS PLC
Inventors: DE COPPI, Paolo
URBANI, Luca
PINZANI, Massimo
MAZZA, Guiseppe
CROWLEY, Claire
Title: CRYOPRESERVATION
Abstract:
The invention provides methods and materials for the cryopreservation of cellularised scaffolds, which may be used for therapeutic or pharmacological testing purposes, which methods comprise: (i) providing a cultured scaffold on which cells have been seeded; (ii) equilibrating said cellularised scaffold with a cryopreservative composition comprising culture medium and between 5 and 30% of a cryoprotectant such as DMSO; (iii) freezing the equilibrated cellularised scaffold by reducing the temperature continuously by about - 1°C/minute to about -80°C; (iv) storing the frozen cellularised scaffold at a temperature of between -135°C and -198°C.