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1. (WO2018219995) YEAST EXPRESSING A SYNTHETIC CALVIN CYCLE

Pub. No.:    WO/2018/219995    International Application No.:    PCT/EP2018/064158
Publication Date: Fri Dec 07 00:59:59 CET 2018 International Filing Date: Thu May 31 01:59:59 CEST 2018
IPC: C12N 1/16
C12N 9/88
C12N 15/81
Applicants: UNIVERSITÄT FÜR BODENKULTUR WIEN
ACIB GMBH
Inventors: MATTANOVICH, Diethard
SAUER, Michael
STEIGER, Matthias
GASSLER, Thomas
GASSER, Brigitte
Title: YEAST EXPRESSING A SYNTHETIC CALVIN CYCLE
Abstract:
A yeast comprising a nucleotide sequence expression system expressing a synthetic Calvin cycle comprising heterologous genes, which include at least a) a gene encoding an enzyme from the class of the ribulose-bisphosphate carboxylases (EC number: 4.1.1.39) (RuBisCO gene); and b) a gene encoding an enzyme from the class of the ribulose phosphate kinases (EC number: 2.7.1.19) (PRK gene), which is expressing; wherein the yeast optionally comprises a heterologous expression construct expressing a gene of interest (GOI) and/or wherein each of said RuBisCO gene and said PRK gene, is fused with a nucleotide sequence encoding a peroxisomal targeting signal (PTS).