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1. (WO2018219978) CONTINUOUS SPECTROMETRIC ASSAYS FOR MEASURING MEPRIN ACTIVITY USING AN AUXILIARY ENZYME

Pub. No.:    WO/2018/219978    International Application No.:    PCT/EP2018/064126
Publication Date: Fri Dec 07 00:59:59 CET 2018 International Filing Date: Thu May 31 01:59:59 CEST 2018
IPC: C12Q 1/68
G01N 33/68
Applicants: FRAUNHOFER-GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
Inventors: SCHLENZIG, Dagmar
HEMPEL, Marvin
DEMUTH, Hans-Ulrich
SCHULZE, Anja
SCHILLING, Stephan
RAMSBECK, Daniel
WERMANN, Michael
Title: CONTINUOUS SPECTROMETRIC ASSAYS FOR MEASURING MEPRIN ACTIVITY USING AN AUXILIARY ENZYME
Abstract:
The present invention relates to assays for measuring meprin activity. More particularly, the assays concern rapid, sensitive, reliable and robust assays for measuring meprin activity comprising a coupled enzyme assay suitable for high-throughput screening. In particular, the invention relates to methods for 1) for assaying meprin catalytic activity; 2) identifying inhibitors of meprin activity; 3) methods for measuring the IC50 of a test compound inhibiting meprin activity; 4) methods for differentiating a competitive inhibitor meprin from a non-competitive inhibitor of meprin; and 5) methods for identifying specific inhibitors for meprin α and meprin β. Further provided is a kit, which can be used to perform the methods of the invention.