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1. (WO2018219969) BIOSENSOR FOR CONFORMATION AND SECONDARY STRUCTURE ANALYSIS

Pub. No.:    WO/2018/219969    International Application No.:    PCT/EP2018/064103
Publication Date: Fri Dec 07 00:59:59 CET 2018 International Filing Date: Wed May 30 01:59:59 CEST 2018
IPC: G01N 33/52
G01N 21/552
G01N 21/3577
G01N 21/64
Applicants: RUHR-UNIVERSITÄT BOCHUM
Inventors: GERWERT, Klaus
Title: BIOSENSOR FOR CONFORMATION AND SECONDARY STRUCTURE ANALYSIS
Abstract:
The invention provides an infrared detection system for conformation and secondary structure analysis, notably for the direct non-invasive qualitative secondary structure analysis of a single selected protein within a complex mixture, as e.g. a body fluid, by vibrational spectroscopic methods utilizing a quantum- cascade laser. For the analysis it is not required that the selected substance be isolated, concentrated, or pretreated by a special preparative procedure. A difference-spectrum between the unbound and antibody-bound protein of interest is performed by which the much larger background absorbance is cancelled. The presented quantum- cascade laser set-up provides sufficient S/N and stability to subtract the several orders of magnitude larger background absorbance.