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1. (WO2018219684) CULTURE MEDIUM FOR PLURIPOTENT STEM CELLS

Pub. No.:    WO/2018/219684    International Application No.:    PCT/EP2018/063036
Publication Date: Fri Dec 07 00:59:59 CET 2018 International Filing Date: Sat May 19 01:59:59 CEST 2018
IPC: C12N 5/074
C12N 5/00
Applicants: PROMOCELL BIOSCIENCE ALIVE GMBH BIOMEDIZINISCHE PRODUKTE
Inventors: WIELAND, Hagen
Title: CULTURE MEDIUM FOR PLURIPOTENT STEM CELLS
Abstract:
The present invention relates to a chemically defined medium for eukaryotic cell culture, comprising water, at least one carbon source, one or more vitamins, one or more salts, one or more fatty acids, one or more buffer components, selenium, at least one substance of the group of Functional Inhibitors of Acid Sphingomyelinase (FIASMAs) and at least one polypeptide of the TGF-β superfamily with the ability to inhibit stem cell differentiation, its use in the culture of human pluripotent stem cells, a cell culture system comprising human pluripotent stem cells and the chemically defined medium, as well as a kit for proliferation and/or maintenance of human pluripotent stem cells.