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1. (WO2018219632) METHOD FOR GENERATING AND ANALYSING AN OVERVIEW CONTRAST IMAGE

Pub. No.:    WO/2018/219632    International Application No.:    PCT/EP2018/062483
Publication Date: Fri Dec 07 00:59:59 CET 2018 International Filing Date: Wed May 16 01:59:59 CEST 2018
IPC: G02B 21/36
G06T 7/586
G02B 21/08
G02B 21/12
Applicants: CARL ZEISS MICROSCOPY GMBH
Inventors: HAASE, Daniel
OHRT, Thomas
STICKER, Markus
Title: METHOD FOR GENERATING AND ANALYSING AN OVERVIEW CONTRAST IMAGE
Abstract:
The invention relates to a method for generating and analysing an overview contrast image of a specimen carrier (1) and/or of specimens situated on a specimen carrier (1). A specimen carrier (1) arranged at least partially in the focus of a detection optical unit (2) is illuminated in transmitted light using a two-dimensional, array-like illumination pattern (3). At least two overview raw images are detected using different illuminations of the specimen carrier (1), and, according to information to be extracted from the overview contrast image, a combination algorithm is selected by means of which the at least two overview raw images are combined to form the overview contrast image. According to information to be extracted from the overview contrast image, an image evaluation algorithm is selected by means of which the information is extracted.