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1. (WO2018219171) BACTERIUM PRODUCING DHA AND EPA, 6 GENE FRAGMENTS OF GENOME OF BACTERIUM AND USE THEREOF

Pub. No.:    WO/2018/219171    International Application No.:    PCT/CN2018/087613
Publication Date: Fri Dec 07 00:59:59 CET 2018 International Filing Date: Tue May 22 01:59:59 CEST 2018
IPC: C07K 14/37
C12N 15/31
C12N 1/15
C12N 1/14
C12P 7/64
C12R 1/645
Applicants: XIAMEN HUISON BIOTECH CO., LTD.
厦门汇盛生物有限公司
Inventors: CHEN, Liyi
陈礼毅
ZHONG, Huichang
钟惠昌
CHEN, Shuirong
陈水荣
Title: BACTERIUM PRODUCING DHA AND EPA, 6 GENE FRAGMENTS OF GENOME OF BACTERIUM AND USE THEREOF
Abstract:
Disclosed are a bacterium producing DHA and/or EPA, 6 gene fragments of the genome of the bacterium and the use thereof. The bacterium is Schizochytrium limacinum HS01 with the deposit number in China General Microbiological Culture Collection Center being CGMCC No.13746. The 6 gene fragments consist of gene fragment 1 to gene fragment 6, and the nucleotide sequences are sequentially as shown in sequence 3 to sequence 8 of the sequencing listing. Experiments prove that, a fermentation broth containing DHA and EPA is obtained through fermentation cultivation of Schizochytrium limacinum HS01; the gene fragments 1-6 are introduced into the Schizochytrium limacinum MYA-1381 to obtain a recombinant bacterium; and the capability of the recombinant bacterium to produce DHA and EPA is greatly improved. The bacterium, 6 gene fragments, proteins encoded by the 6 gene fragments, and a vector, cell or organism containing the 6 gene fragments provided by the present invention all have an important application value.