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1. (WO2018219093) METHOD FOR CONSTRUCTING GLRX1 GENE KNOCK-OUT ANIMAL MODEL BASED ON CRISPR/CAS9
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Pub. No.: WO/2018/219093 International Application No.: PCT/CN2018/085432
Publication Date: 06.12.2018 International Filing Date: 03.05.2018
IPC:
C12N 15/90 (2006.01) ,C12N 9/22 (2006.01) ,A01K 67/027 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
87
Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
90
Stable introduction of foreign DNA into chromosome
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
16
acting on ester bonds (3.1)
22
Ribonucleases
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
K
ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
67
Rearing or breeding animals, not otherwise provided for; New breeds of animals
027
New breeds of vertebrates
Applicants:
南京农业大学 NANJING AGRICULTURAL UNIVERSITY [CN/CN]; 中国江苏省南京市 玄武区卫岗1号 No.1 Weigang, Xuanwu Nanjing, Jiangsu 210095, CN
Inventors:
李春保 LI, Chunbao; CN
周光宏 ZHOU, Guanghong; CN
邹小雨 ZOU, Xiaoyu; CN
石学彬 SHI, Xuebin; CN
徐幸莲 XU, Xinglian; CN
Agent:
南京天华专利代理有限责任公司 NANJING TIANHUA PATENT AGENT CO., LTD.; 中国江苏省南京市 鼓楼区山西路67号世贸中心大厦A1座23层 Floor 23, Block A1, World Trade Center Building, No. 67 Shanxi Road, Gulou Nanjing, Jiangsu 210009, CN
Priority Data:
201710390894.327.05.2017CN
Title (EN) METHOD FOR CONSTRUCTING GLRX1 GENE KNOCK-OUT ANIMAL MODEL BASED ON CRISPR/CAS9
(FR) MÉTHODE DE CONSTRUCTION D'UN MODÈLE ANIMAL KNOCK-OUT DE GÈNE GLRX1 BASÉ SUR CRISPR/CAS9
(ZH) 一种基于CRISPR/Cas9技术的Glrx1基因敲除动物模型的构建方法
Abstract:
(EN) The invention discloses a method for constructing a Glrx1 gene knock-out animal model based on CRISPR/Cas9, comprising the steps of: 1. selection and design of sgRNA targeting Glrx1 gene of mice; 2. construction of an sgRNA vector; 3. in vitro transcription of sgRNA; 4. injection of mouse fertilized eggs; 5. birth and identification of F0-generation mice; and 6. breeding of positive F0-generation mice, and birth and identification of F1-generation mice.
(FR) L'invention concerne une méthode de construction d'un modèle animal knock-out de gène Glrx1 basé sur CRISPR/Cas9, comprenant les étapes consistant à : 1. sélection et conception de sgARN ciblant le gène Glrx1 de souris ; 2. construction d'un vecteur de sgARN ; 3. transcription in vitro de sgARN ; 4. injection d’œufs fécondés de souris ; 5. naissance et identification de souris de génération F0 ; et 6. sélection de souris de génération F0 positives, et naissance et identification de souris de génération F1.
(ZH) 一种基于CRISPR/Cas9技术的Glrx1基因敲除动物模型的构建方法,包括以下步骤:一、靶向小鼠Glrx1基因的sgRNA的选择和设计,二、sgRNA载体构建,三、sgRNA体外转录,四、小鼠受精卵注射,五、F0代小鼠出生和鉴定,六、阳性F0代小鼠配繁,F1代小鼠出生与鉴定。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)