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1. (WO2018218510) KIT AND METHOD FOR LABELING BACTERIAL FLORA, FLUORESCENCE-LABELED BACTERIAL FLORA AND APPLICATION THEREOF
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Pub. No.: WO/2018/218510 International Application No.: PCT/CN2017/086618
Publication Date: 06.12.2018 International Filing Date: 31.05.2017
IPC:
G01N 21/00 (2006.01) ,G01N 33/569 (2006.01) ,C12Q 1/04 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21
Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible, or ultra-violet light
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
569
for micro-organisms, e.g. protozoa, bacteria, viruses
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
04
Determining presence or kind of micro-organism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
Applicants:
北京大学 PEKING UNIVERSITY [CN/CN]; 中国北京市 海淀区颐和园路5号 No.5 Yiheyuan Road, Haidian District Beijing 100871, CN
Inventors:
陈兴 CHEN, Xing; CN
王炜 WANG, Wei; CN
Agent:
北京康信知识产权代理有限责任公司 KANGXIN PARTNERS, P.C.; 中国北京市 海淀区知春路甲48号盈都大厦A座16层 Floor 16, Tower A, Indo Building A48 Zhichun Road, Haidian District Beijing 100098, CN
Priority Data:
Title (EN) KIT AND METHOD FOR LABELING BACTERIAL FLORA, FLUORESCENCE-LABELED BACTERIAL FLORA AND APPLICATION THEREOF
(FR) TROUSSE ET PROCÉDÉ DE MARQUAGE DE LA FLORE BACTÉRIENNE, FLORE BACTÉRIENNE MARQUÉE PAR FLUORESCENCE ET APPLICATION ASSOCIÉE
(ZH) 标记细菌菌群的试剂盒、方法、带荧光标记的细菌菌群及其应用
Abstract:
(EN) Provided in the present invention are a kit and method for labeling bacterial flora, fluorescence-labeled bacterial flora and an application thereof. The method comprises: using a first type of fluorescence probe and a second type of fluorescence probe to label bacterial species in bacterial flora, wherein the first type of fluorescence probe specifically labels Gram-positive bacteria, and the second type of fluorescence probe specifically labels Gram-negative bacteria, the first type of fluorescence probe and the second type of fluorescence probe carrying different fluorescence markers, respectively.
(FR) La présente invention concerne une trousse et un procédé de marquage de la flore bactérienne, de la flore bactérienne marquée par fluorescence et une application associée. Le procédé comprend : l'utilisation d'un premier type de sonde de fluorescence et d'un second type de sonde de fluorescence pour marquer des espèces bactériennes dans une flore bactérienne, le premier type de sonde de fluorescence marquant spécifiquement des bactéries à Gram positif, et le second type de sonde de fluorescence marquant spécifiquement des bactéries à Gram négatif, le premier type de sonde de fluorescence et le second type de sonde de fluorescence portant différents marqueurs de fluorescence, respectivement.
(ZH) 本发明提供了一种标记细菌菌群样品的试剂盒、方法、带荧光标记的细菌菌群及其应用。所述方法包括利用第一类荧光探针和第二类荧光探针对细菌菌群中的菌种进行标记,其中第一类荧光探针特异性标记革兰氏阳性菌,第二类荧光探针特异性标记革兰氏阴性菌,且第一类荧光探针和第二类荧光探针分别带有不同的荧光标记。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)