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1. (WO2018205165) SCHIZOCHYTRIUM LIMACINUM STRAIN, BUILDING METHOD THEREFOR AND APPLICATION THEREOF
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Pub. No.: WO/2018/205165 International Application No.: PCT/CN2017/083764
Publication Date: 15.11.2018 International Filing Date: 10.05.2017
IPC:
C12N 1/15 (2006.01) ,C12P 7/64 (2006.01) ,C12R 1/645 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
14
Fungi; Culture media therefor
15
modified by introduction of foreign genetic material
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7
Preparation of oxygen-containing organic compounds
64
Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
R
INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1
Micro-organisms
645
Fungi
Applicants:
南京工业大学 NANJING UNIVERSITY OF TECHNOLOGY [CN/CN]; 中国江苏省南京市 浦口区浦珠南路30号 30# Puzhu South Road, Pukou District Nanjing, Jiangsu 211816, CN
Inventors:
黄和 HUANG, He; CN
任路静 REN, Lujing; CN
陈胜兰 CHEN, Shenglan; CN
耿灵君 GENG, Lingjun; CN
纪晓俊 JI, Xiaojun; CN
胡学超 HU, Xuechao; CN
Agent:
江苏致邦律师事务所 JIANGSU CO-FAR LAW FIRM; 中国江苏省南京市 鼓楼区石头城6号文化产业园05幢江苏致邦律师事务所徐蓓 c/o XU, Pei Jiangsu Co-Far Law Firm, Block 5, Cultural Industrial Park 6#, Shitoucheng Road, Gulou District Nanjing, Jiangsu 210013, CN
Priority Data:
Title (EN) SCHIZOCHYTRIUM LIMACINUM STRAIN, BUILDING METHOD THEREFOR AND APPLICATION THEREOF
(FR) SOUCHE SCHIZOCHYTRIUM LIMACINUM, SA MÉTHODE DE CONSTRUCTION ET SON APPLICATION
(ZH) 一株裂殖壶菌菌株、其构建方法及应用
Abstract:
(EN) Provided is a Schizochytrium limacinum strain, a building method therefor and an application thereof. The strain disclosed is classified and named as Schizochytrium sp. HX-RS, and the preservation number is CCTCC NO: M2017046. An acyltransferase functional domain originating from shewanella PKS enzyme is adopted instead of an acyltransferase functional domain originating from schizochytrium sp. PKS enzyme, and the strain is obtained by performing flat panel screening and acclimation screening with a high rotation speed and a low temperature.
(FR) L'invention concerne une souche de Schizochytrium limacinum, sa méthode de construction et son application. La souche décrite est classée et nommée Schizochytrium sp. HX-RS, et le numéro de conservation est CCTCC NO: M2017046. Un domaine fonctionnel d'acyltransférase provenant d'une enzyme PKS shewanella est adopté à la place d'un domaine fonctionnel d'acyltransférase provenant d'une enzyme PKS schizochytrium sp. , et la souche est obtenue par réalisation d'un criblage de panneau plat et d'un criblage d'acclimatation avec une vitesse de rotation élevée et une basse température.
(ZH) 提供了一株裂殖壶菌菌株、其构建方法及应用,该菌株的分类命名为裂殖壶菌(Schizochytrium sp.)HX-RS,保藏编号CCTCC NO:M2017046。采用来自希瓦氏菌PKS酶中的酰基转移酶功能域替换裂殖壶菌PKS酶中的酰基转移酶功能域,并通过平板筛选、高转速低温驯化筛选获得该菌株。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)